| RAPD在玉米品种鉴定和纯度分析中的应用 |
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| 引用本文： | 吴敏生,戴景瑞.RAPD在玉米品种鉴定和纯度分析中的应用[J].作物学报,1999,25(4):489-493. |
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| 作者姓名： | 吴敏生 戴景瑞 |
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| 作者单位： | 中国农业大学植物遗传育种系，北京，100094 |
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| 基金项目： | 国家自然科学基金和国家“九五”科技攻关项目资助 |
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| 摘 要： | 以7个优良玉米自交系和它们的7个单交种为材料，从种子中提取DNA，进行RAPD扩增，探索利用RAPD标记鉴定玉米品种和纯度分析的可能性。研究结果表明，自交系间、杂交种间、自交系和杂交种间均有明显差异，利用3个引物可将14个材料完全分开。RAPD技术用于玉米品种鉴定和纯度分析是可行的。
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| 关 键 词： | RAPD 玉米 品种鉴定 纯度分析 |
| 收稿时间： | 1999-01-06 |
| 修稿时间： | 1999-05-16 |
Application of RAPD in Cultivar Identification and Purity Test in Maize |
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| Wu Minsheng,Dai Jingrui,Wang Shoucai.Application of RAPD in Cultivar Identification and Purity Test in Maize[J].Acta Agronomica Sinica,1999,25(4):489-493. |
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| Authors: | Wu Minsheng Dai Jingrui Wang Shoucai |
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| Institution: | Department of Genetic and Breeding, China Agricultural University, Beijing, 100094 |
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| Abstract: | DNA extracted from maize seeds of seven elite inbred lines and seven of their single-crosses was analyzed with RAPD technique. The results showed that there were obvious differences between different inbred lines, between different hybrids, and between inbred lines and hybrids. Fourteen maize materials could easily be differentiated by using three primers. It is feasible, therefore, to identify maize cultivars and their purity with the RAPD technique. |
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| Keywords: | Random amplified polymorphic DNA Maize Cultivar identification Purity test |
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