| 除草剂抗性基因bar导人烟草叶绿体 |
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| 引用本文： | 张中林,钱凯先.除草剂抗性基因bar导人烟草叶绿体[J].作物学报,1999,25(5):574-578. |
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| 作者姓名： | 张中林 钱凯先 |
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| 作者单位： | 中国农业科学院生物技术研究中心，北京，100081 |
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| 基金项目： | 本研究系国家科委863专项基金资助 |
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| 摘 要： | 将编码Phosphinothricin acetyltrans ferase的bar基因与水稻叶绿体psbA基因的启动子和终止子构建成表达盒，连同烟草叶绿体基因组同源片段rpl2-trnH-psbA和trnK-ORF509A以及选择标记基因aadA一起构建成烟草叶绿体转化载体pTZBA。基因枪法转化烟草叶片，经壮观霉素筛选获得转化再生植株。分别以1.0 kb的叶绿体同源片段trnK-OR
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| 关 键 词： | bar基因 叶绿体转化 同源片段 烟草 aadA基因 |
| 收稿时间： | 1999-01-10 |
| 修稿时间： | 1999-07-18 |
Herbicide Resistance Gene (bar) was Introduced into the Chloroplast of Tobacco |
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| Zhang Zhonglin Shan Song Chen Xi Liu Chunying Qian Kaixian Shen Guifang.Herbicide Resistance Gene (bar) was Introduced into the Chloroplast of Tobacco[J].Acta Agronomica Sinica,1999,25(5):574-578. |
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| Authors: | Zhang Zhonglin Shan Song Chen Xi Liu Chunying Qian Kaixian Shen Guifang |
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| Institution: | Biotechnology Research Center；Chinese Academy Academy of Agricultural Sciences，Beijing 100081 |
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| Abstract: | The herbicide resistance gene bar encoding Phosphinothricin acetyltransferase was placed under the control of psbA5' and psbA3' of rice to construct bar cassette, which was ligat-ed with the selectable marker aadA gene and these recombined fragments located between the two homology region of tobacco chloroplast genome to generate transformation vector pTZBA. Selection was for spectinomycin resistance after biolistic delivery of plasmid pTZBA DNA into leaf cells. DNA gel-blot analysis probed with homology region trnK-ORF509A and bar confirmed incorporation of the chimeric bar gene into the plastid genome by homologous recombination events via the flanking plastid gene sequences. Some transgenic plants showed resistant to PPT in the test. |
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| Keywords: | bar gene Chloroplast transformation Homologous fragment Tobacco aad A gene |
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