| 利用nrDNAITS探讨雄性不育“YX-1”与宁夏枸杞的亲缘关系 |
| |
| 引用本文： | 石志刚,安巍,曹有龙.利用nrDNAITS探讨雄性不育“YX-1”与宁夏枸杞的亲缘关系[J].北方园艺,2009(3). |
| |
| 作者姓名： | 石志刚 安巍 曹有龙 |
| |
| 作者单位： | 宁夏枸杞工程技术研究中心,宁夏,银川,750002 |
| |
| 摘 要： | 采用改进CTAB法提取枸杞叶片DNA,利用合成的特异引物对其DNA中nrDNA ITS区进行扩增、克隆,对目的片段测序分析.利用nrDNA ITS(核核糖体DNA基因内转录间隔区)序列,探讨枸杞雄性不育材料"YX-1"与其它7份宁夏枸杞材料的亲缘关系.结果表明:首次测序得到了枸杞雄性不育材料和其它7份枸杞种质的nrDNA ITS区碱基序列,整个ITS序列长度变异范围为559～633 bp,平均为612 bp,初步从基于ITS序列得出的NJ聚类图可以判断枸杞雄性不育"YX-1"在DNA水平与其他7个枸杞品种存在差异,其与四倍体88024亲缘关系较近.基于nrDNA ITS区序列分析可作为探讨枸杞雄性不育材料"YX-1"亲缘关系的一种新方法.
|
| 关 键 词： | 枸杞属 ITS序列 DNA测序 鉴定 |
Study on the Genetic Relationships of Male Sterility in Lycium linn and Lycium barbarum L. by nrDNA ITS Sequence |
| |
| SHI Zhi-gang,AN Wei,CAO You-long.Study on the Genetic Relationships of Male Sterility in Lycium linn and Lycium barbarum L. by nrDNA ITS Sequence[J].Northern Horticulture,2009(3). |
| |
| Authors: | SHI Zhi-gang AN Wei CAO You-long |
| |
| Institution: | Ningxia Wolfberry Engineering and Technology Research Center;Yinchuan;Ningxia 750002;China |
| |
| Abstract: | Genomic DNA from wolfberry leaves extracted by modified CTAB method were regarded as templates for PCR amplification by synthetic specific primer,cloning and sequencing.The nrDNA ITS sequence of male sterility "YX-1" and other 7 Lycium barbarum L.measured in Lycium Linn were investigated.The study aimed to identify the genetic relationships of Male sterility in Lycium linn.and Lycium barbarum L.Result showed that the nrDNA ITS sequence of male sterility "YX-1" and other 7 germplasms measured in Lycium Linn ... |
| |
| Keywords: | Lycium Linn Internal transcribed spacer Measure the sequence of DNA Identification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
