| 桃色顶孢霉发酵液对大豆的促生及其抗逆酶活性的影响 |
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| 引用本文： | 张雨竹,董雪梅,郭春兰,于春生,孙冬梅﹡.桃色顶孢霉发酵液对大豆的促生及其抗逆酶活性的影响[J].中国油料作物学报,2014,36(4):519. |
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| 作者姓名： | 张雨竹 董雪梅 郭春兰 于春生 孙冬梅﹡ |
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| 作者单位： | 黑龙江八一农垦大学，黑龙江 大庆 163319 |
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| 基金项目： | 本研究由黑龙江省教育厅科研资助项目(面上)(12511361) 资助 |
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| 摘 要： | 本实验采用了管碟法和种子萌发法研究了实验室分离获得的桃色顶孢霉对茄病镰刀菌F. solani.的拮抗作用以及对大豆的促生作用，并采用愈创木酚法、紫外吸收法、氮蓝四唑光化还原法研究了顶孢霉发酵液对POD、CAT、SOD三种抗逆酶活性的影响。结果表明：顶孢霉发酵液对镰刀菌菌丝生长有明显抑制作用，菌丝变稀疏。大豆种子经不同浓度桃色顶孢霉发酵液处理后，萌发率及种子酶活性均产生变化。不同浓度的发酵液对种子萌发促进作用不同，当发酵液浓度为10%时，胚根长，胚芽长，胚根鲜重，胚芽鲜重均达到最高值，分别为2.91cm，0.037g，4.07cm，0.1666g。与对照相比分别提高了49.37%，63.24%，30.54%，45.80%。当处理种子的发酵液浓度为60%时，种子萌发率最高为83.67%，与对照相比提高了23.90%；酶活性最强，POD、CAT和SOD分别为683U、160.67U和301U，与对照相比分别提高 73.50%，43.78%，76.73%。初步证实了桃色顶孢霉发酵液中含有能够促进大豆种子的萌发及抗逆酶活性升高的物质。
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| 关 键 词： | 桃色顶孢霉 发酵液 种子萌发 抗逆酶活性 |
Effect of Acremonium persicium fermentation on seeds germination and antioxidant enzymes activity of soybean |
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| ZHANG Yu-zhu,DONG Xue-mei,GUO Chun-lan,YU Chun-sheng,SUN Dong-mei.Effect of Acremonium persicium fermentation on seeds germination and antioxidant enzymes activity of soybean[J].Chinese Journal of Oil Crop Sciences,2014,36(4):519. |
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| Authors: | ZHANG Yu-zhu DONG Xue-mei GUO Chun-lan YU Chun-sheng SUN Dong-mei |
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| Abstract: | In this study, the antagonism ability of fermentation of Acremonium persicinum to Fusarium solanae and the growth promoting ability on soybean germination process were studied by cylinder-plate method and seed germination method. Guaiacol, UV spectrophotometric , NBT-law were used to determine POD, CAT, SOD of soybean seed to evaluate effects of fermentation of A. persicium. The result showed that the hypha growth of F. solanae was inhibited by the fermentation of A. persicinum, and the hypha became less dense. The germination rate and enzyme activity of soybean seeds treated by different concentration of fermentation were all changed. The affectivity were different, when treated by the fermentation at the concentration of 10%, the length of radicle and hypocotyl, the raw weight of radicle and hypocotyl were all at the highest value, increased by 49.37%, 63.24%, 30.54% and 45.80% respectively, comparing to control. When treated by the fermentation at the concentration of 60%, the germination rate was the highest, up to 83.7%; the POD, CAT and SOD enzyme activities were 683U, 160.67U and 301U respectively, increased by 73.50%, 43.78% and 76.73%, comparing to control. It was confirmed that there were some constituents could promote soybean seeds germination and increase anti-stress enzyme activities in the A. persicinum fermentation. |
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| Keywords: | Acremonium persiciun Fermentation Seed germination Anti-stress enzyme activity |
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