| 花生中UDP-glucosyltransferase基因的克隆及在非生物胁迫下的表达研究 |
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| 引用本文： | 陈 娜,胡东青,潘丽娟,迟晓元,陈明娜,王 通,王 冕,杨 珍,禹山林.花生中UDP-glucosyltransferase基因的克隆及在非生物胁迫下的表达研究[J].中国油料作物学报,2014,36(3):308. |
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| 作者姓名： | 陈 娜 胡东青 潘丽娟 迟晓元 陈明娜 王 通 王 冕 杨 珍 禹山林 |
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| 作者单位： | 1. 山东省花生研究所，山东 青岛 266100；2. 山东省青岛市出入境检验检疫局，山东 青岛 266001 |
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| 基金项目： | 国家产业体系（CARS-14）；山东省自然基金（ZR2011CQ036; ZR2012CQ031）；国家自然基金（31000728; 31100205; 31200211）；山东省优秀中青年科学家科研奖励基金（BS2010NY023）；青岛市科技计划（11-2-4-9-(3)-jch; 11-2-3-26-nsh; 12-1-4-11-(2)-jch） |
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| 摘 要： | 通过RACE技术从花生叶片cDNA中克隆到了UDP-glucosyltransferase基因，命名为AhUGT83A1-like （Genbank注册号为KF411463）。该基因全长为1 530bp，ORF为1 380bp，编码460个氨基酸。序列比对与进化分析结果表明，该序列有保守的UDPGT结构域，并且与其它植物的UGT蛋白同源性较高。荧光定量PCR结果表明，AhUGT83A1-like基因在高盐和低温处理的花生根和叶片中均上调表达；在干旱处理时，根中表达也受到显著上调，但叶片中表达量则有明显下降。以上结果表明AhUGT83A1-like可能参与了花生对干旱、低温和高盐的抗性调控。ABA处理结果表明，AhUGT83A1-like在花生根中对ABA响应明显，但在叶片中对ABA没有明显响应，表明该基因在花生根中对非生物胁迫的调控可能是以依赖ABA的方式发挥作用的。?
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| 关 键 词： | 花生 AhUGT83A1-like 基因克隆 非生物胁迫 荧光定量PCR。 |
Cloning of UDP-glucosyltransferase gene from peanut (Arachis hypogaea L.) and its expression analysis during abiotic stress |
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| CHEN Na,HU Dong-qing,PAN Li-juan,CHI Xiao-yuan,CHEN Ming-na,WANG Tong,WANG Mian,YANG Zhen,YU Shan-lin.Cloning of UDP-glucosyltransferase gene from peanut (Arachis hypogaea L.) and its expression analysis during abiotic stress[J].Chinese Journal of Oil Crop Sciences,2014,36(3):308. |
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| Authors: | CHEN Na HU Dong-qing PAN Li-juan CHI Xiao-yuan CHEN Ming-na WANG Tong WANG Mian YANG Zhen YU Shan-lin |
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| Abstract: | UDP-glucosyltransferase gene was cloned from peanut leaf using RACE technology and was named AhUGT83A1-like (GenBank accession KF411463). The full length cDNA of AhUGT83A1-like was 1 530bp, ORF 1 380bp, encoding 460 amino acids. Sequence alignment and phylogenetic analysis revealed that AhUGT83A1-like had the conserved UDPGT domain and shared high homology with UGT proteins from other plant species. Expression analysis indicated that AhUGT83A1-like was induced in peanut roots and leaves under both cold and salt conditions. The expression of AhUGT83A1-like was increased in drought-treated roots, but decreased in drought-treated leaves. The results suggested AhUGT83A1-like may be involved in abiotic stress regulation of peanut. In addition, AhUGT83A1-like was induced by ABA in peanut roots, which indicated that AhUGT83A1-like protein might have participated in abiotic stress regulation in the ABA-dependent pathway in peanut roots. |
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| Keywords: | Peanut AhUGT83A1-like Gene clone Abiotic stress Real-time PCR |
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