| 缺氧和有毒微囊藻胁迫下三角帆蚌鳃和主要消化器官以及晶杆体的扫描电镜观察 |
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| 引用本文： | 顾叶丹,尚跃勇,袁明哲,陈雪英,周作强,胡梦红.缺氧和有毒微囊藻胁迫下三角帆蚌鳃和主要消化器官以及晶杆体的扫描电镜观察[J].水产学报,2016,40(9):1486-1496. |
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| 作者姓名： | 顾叶丹 尚跃勇 袁明哲 陈雪英 周作强 胡梦红 |
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| 作者单位： | 上海海洋大学水产与生命学院,上海,201306 |
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| 基金项目： | 国家自然科学基金（31202015）；上海市高校知识服务平台项目（ZF1206） |
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| 摘 要： | 为了阐明缺氧和有毒铜绿做囊藻对三角帆蚌鳃和主要消化器官的毒理效应和组织病理,实验利用扫描电镜研究了暴露于缺氧和有毒铜绿做囊藻下三角帆蚌的鳃、胃、肠以及晶杆体等器官组织病理学变化。结果显示,从第7天开始缺氧和有毒藻类复合胁迫组三角帆蚌的鳃丝及柱状细胞出现大量坏死和脱落,胃肠腔面纤毛脱落、上史细胞破裂坏死,晶杆体在第5天已经完全消失;第7天单独缺氧组和单独有毒藻类组的鳃和消化道出现少量的病变,单独缺氧组晶杆体在第7天完全消失,单独有毒藻类组晶杆体则一直存在。胁迫消失后,3个实验暴露组的晶杆体都未恢复正常对照组水平,因此缺氧和有毒铜绿做囊藻对三角帆蚌鳃和消化系统产生不可恢复性损伤,双重胁迫组影响最为严重,缺氧胁迫组相对有毒铜绿做囊藻胁迫组影响更为显著。本研究为三角帆蚌在缺氧和有毒藻类胁迫下的生理适应机制提供了组织病理学上的参考,并为其作为富营养化水体治理工具种的可行性提供了理论依据。
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| 关 键 词： | 三角帆蚌 有毒铜绿微囊藻 晶杆体 鳃结构 缺氧 扫描电镜 |
| 收稿时间： | 2015/4/22 0:00:00 |
| 修稿时间： | 7/5/2016 12:00:00 AM |
Scanning electron microscopic observations of gill, digestive organ and crystal rod of Hyriopsis cumingii exposed to hypoxia and toxic Microcystis aeruginosa |
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| GU Yedan,SHANG Yueyong,YUAN Mingzhe,CHEN Xueying,ZHOU Zuoqiang and HU Menghong.Scanning electron microscopic observations of gill, digestive organ and crystal rod of Hyriopsis cumingii exposed to hypoxia and toxic Microcystis aeruginosa[J].Journal of Fisheries of China,2016,40(9):1486-1496. |
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| Authors: | GU Yedan SHANG Yueyong YUAN Mingzhe CHEN Xueying ZHOU Zuoqiang and HU Menghong |
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| Institution: | College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China,College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China,College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China,College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China,College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China and College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China |
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| Abstract: | In order to clarify the toxic effects of hypoxia and toxic Microcystis aeruginosa on freshwater pearl mussel (Hygriopsis cumingii) and histopathology, scanning electron microscopy was used to investigate the histopathological changes in the gill, stomach, intestine and crystal rod of the freshwater pearl mussel. The results showed that a large number of gill shedding and columnar cell necrosis were observed from the seventh day in the combined experimental group with hypoxia and toxic algae, as well as cilia loss from gastrointestinal cavity surface and epithelial cell necrosis rupture, and the crystal rod completely disappeared from the fifth day; from the seventh day, in hypoxia group and toxic algae group, a small amount of gill and digestive tract lesions were observed, and in hypoxia group, crystal rod completely disappeared from the seventh day, while in toxic algae group, the crystal rod persists throughout the experiment. After the stress was removed, in the three exposed groups, the crystal rods did not return to the normal levels as in the control group. Therefore, hypoxia and toxic Microcystis aeruginosa caused unrecoverable damages in gill and digestive system of H. cumingii, and the combined stress group was most severely affected, and hypoxia caused more severe effects than the toxic Microcystis aeruginosa. This study provides a histopathological reference for the physiological adaptation mechanisms in H. cumingii exposed to hypoxia and toxic algae, and a theoretical basis for the feasibility of H. cumingii as a bioremediation tool species for improving eutrophic waters. |
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| Keywords: | Hyriopsis cumingi toxic Microcystis aeruginosa crystalline style gill structure hypoxia scanning electron microscopy |
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