| 锥栗不同品种遗传距离的RAPD 分析 |
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| 引用本文： | 雷日平,陈辉,谢利国.锥栗不同品种遗传距离的RAPD 分析[J].浙江农林大学学报,2002,19(3):240-243. |
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| 作者姓名： | 雷日平 陈辉 谢利国 |
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| 作者单位： | 1.浙江林学院 科研处, 浙江 临安311300;2.福建农林大学 作物学院, 福建 福州 353000;3.浙江省泰顺县乌岩岭自然保护区, 浙江 泰顺 325500 |
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| 摘 要： | 运用RAPD 法对不同锥栗品种间基于DNA 基础上的遗传距离进行了研究, 建立了锥栗PCR 反应的标准程序, 得到的扩增片段大小在500 ～ 2 000 bp 之间, 确定了10 个品种基于DNA 分子水平上的相似系数。当相似系数等于0.74 时, 可把这10 个品种分为4 类, 即油栗仔, 蔓榛, 中尖嘴, 猴嘴榛 红紫榛, 大尖嘴, 材榛 仁嘴栗, 乌棒 和嫁接毛榛 。图2 表2 参6
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| 关 键 词： | 锥栗 随机扩增多态性DNA 遗传距离 |
Analysis of the genetic distance of Castanea henryi with RAPD markers |
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| LEI Ri-ping,CHEN Hui,XIE Li-guo.Analysis of the genetic distance of Castanea henryi with RAPD markers[J].Journal of Zhejiang A&F University,2002,19(3):240-243. |
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| Authors: | LEI Ri-ping CHEN Hui XIE Li-guo |
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| Institution: | 1..Science and Technology Office , Zhejiang Forestry College , Lin'an 311300, Zhejiang , China;2.Crop Institute, Fujian Agriculture and Forestry University , Fuzhou 353000 , Fujian, China;3..Nature Reserve of Wuyanling , Taishun 325500 , Zhejiang , China |
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| Abstract: | The genetic distance of 10 Castanea henryi variances is studies by RAPD (Random Amplified Polymorphic DNA)markers in the paper .The RAPD standard procedure is established , too .The length of amplified fragment is between 500 ～ 2 000 bp after PCR (Polymerase Chain Reaction);and similar coefficient on
the level of DNA molecule is carried out , when the coefficient is 0.74 , 10 variances is divided into four classes , that is , You Lizi , Man Zhen , Zhong Jianzui , Hou Zuizhen , Hong Zizhen , Da Jianzui , Cai Zhen , Ren Zuili , Wu Zhen , and Jiajie Maozhen . |
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