| 兴安落叶松基本群体与育种群体RAPD多样性分析 |
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| 引用本文： | 张振,张含国,张磊,朱航勇,李雪峰.兴安落叶松基本群体与育种群体RAPD多样性分析[J].浙江林学院学报,2012,29(1):130-136. |
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| 作者姓名： | 张振 张含国 张磊 朱航勇 李雪峰 |
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| 作者单位： | 东北林业大学林木遗传育种与生物技术国家重点实验室,黑龙江哈尔滨,150040 |
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| 基金项目： | 国家科技基础性工作专项（2007FY110400-3） |
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| 摘 要： | 利用随机扩增多态性DNA(RAPD)分子标记并结合表型性状对来自乌伊岭、甘河、库都尔、嫩江、莫尔道嘎、阿尔山等6个兴安落叶松Larix gmelinii基本群体的270个样本进行遗传多样性分析.从450个RAPD引物中筛选出23个条带清晰、多态性高的引物,共检测出186个条带,其中182个为多态性条带,多态位点百分率为97.85％,各群体多态位点百分率(PPB)为87.10％～94.09％,所有群体的基因多样度指数(Ht)为0.374 3,群体内基因多样性(Hs)为0.322 6,群体间遗传多样性为0.051 7,86.19％的遗传变异存在于群体内,13.81％的遗传变异存在于群体之间,Shannon指数平均值为0.482 1,Nei's指数平均值为0.322 6;通过对6个群体聚类分析,以0.09的遗传距离可将6个群体聚为3个类群,为小兴安岭分布区、大兴安岭西北部分布区、大兴安岭南部分布区,遗传距离与地理距离存在显著正相关;兴安落叶松4个群体的生长性状表明,树高、胸径、材积遗传变异系数分别为10.25％～20.78％,14.93％～28.37％,35.03％～74.36％,存在着丰富的变异.同兴安落叶松育种群体的遗传变异比较,多态位点百分率、等位变异标记数、Nei's指数、Shannon指数分别高出5.32％,1.13％,4.94％,5.68％,群体总遗传变异高出8.78％,表明兴安落叶松基本群体的遗传变异水平、遗传多样性均高于育种群体,基本群体在群体间的遗传分化要高于育种群体,两者都证实遗传变异主要存在于群体内.
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| 关 键 词： | 林木育种学 兴安落叶松 基本群体 育种群体 随机扩增多态性DNA 生长性状 遗传多样性 |
Diversity analysis with RAPD for natural and breeding populations of Larix gmelinii |
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| ZHANG Zhen
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ZHANG Han-guo
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ZHANG Lei
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ZHU Hang-yong
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LI Xue-feng.Diversity analysis with RAPD for natural and breeding populations of Larix gmelinii[J].Journal of Zhejiang Forestry College,2012,29(1):130-136. |
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| Authors: | ZHANG Zhen ZHANG Han-guo ZHANG Lei ZHU Hang-yong LI Xue-feng |
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| Institution: | (State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin 150040, Heilongjiang, China) |
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| Abstract: | Random amplified polymorphic DNA（RAPD） markers associated with phenotypic characteristics were used to analyze the genetic variation of 270 individual samples from six natural populations of Larix gmelinii（Wuyiling,Ganhe,Kuduer,Nenjiang,Moerdaoga,and Aershan）.From 450 primers,23 clear RAPD primers were selected as their amplified bands with high polymorphism.The analysis included the gene diversity index,Shannon＇s diversity index（SI）,Nei′s gene diversity index,a cluster analysis,and a correlation analysis.Results showed 182 loci among a total of 186 were polymorphic or a percentage of polymorphism（PPB） of 97.9% having a range of 87.10%-94.09%.The gene diversity index was 0.374 3,and the genetic diversity within populations was 0.051 7 indicating that genetic variation was mainly within the population（86.19%）.Mean SI was 0.482 1,and the mean for Nei′s index was 0.322 6.The cluster analysis revealed six populations divided into three groups（the Xiaoxing＇an Mountain Range,northwest of the Daxing＇an Mountain Range,and south of the Daxing＇an Mountain Range） with a genetic distance of 0.09.Genetic distance was significantly and positively correlated with geographical distance（P0.05,r=0.768）.Growth traits of the four L.gmelinii populations were 10.25%-20.78% for height,14.93%-28.37% for diameter,and 35.03%-74.36% for volume coefficients of variation.Compared with the genetic variation of breeding populations,in natural populations PPB was 5.32% greater,mean of effective alleles numbered 1.13% greater,Nei′s index was 4.94% greater,SI was 5.68% greater,and the populations＇ total genetic variation 8.78% greater. |
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| Keywords: | forest tree breeding Larix gmelinii natural population breeding population RAPD growth traits genetic diversity |
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