| 铺地竹试管快速繁殖研究 |
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| 引用本文： | 张有珍,刘倩倩,何冬冬,樊琳,宋李玲,林新春.铺地竹试管快速繁殖研究[J].浙江林学院学报,2012,29(1):151-154. |
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| 作者姓名： | 张有珍 刘倩倩 何冬冬 樊琳 宋李玲 林新春 |
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| 作者单位： | 1. 浙江省临安市林业科学研究所,浙江临安,311300
2. 浙江农林大学亚热带森林培育国家重点实验室培育基地,浙江临安,311300 |
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| 基金项目： | 浙江省科学技术重大专项重点项目（2006C12008） |
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| 摘 要： | 以铺地竹Sasa argenteastriatus侧芽为材料,研究其试管繁殖技术.结果表明:铺地竹最佳增殖培养基为MS(Murashige and Skoog)+3.0 mg· L-1BA (6-苄基腺嘌呤),增殖系数为5.26;最佳生根培养基为MS+5.0 mg· L-1IBA(吲哚丁酸)；试管苗移栽到泥炭、蛭石、珍珠岩体积配制比例为1∶1∶1的基质中,成活效果高达95％.
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| 关 键 词： | 植物学 铺地竹 组织培养 |
In vitro micropropagation of Sasa argenteastriatus |
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| ZHANG You-zhen
,
LIU Qian-qian
,
HE Dong-dong
,
FAN Lin
,
SONG Li-ling
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LIN Xin-chun.In vitro micropropagation of Sasa argenteastriatus[J].Journal of Zhejiang Forestry College,2012,29(1):151-154. |
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| Authors: | ZHANG You-zhen LIU Qian-qian HE Dong-dong FAN Lin SONG Li-ling LIN Xin-chun |
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| Institution: | 1. Forestry Institute of Lin＇an City, Lin＇an 311300, Zhejiang, China; 2. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A ＆ F University, Lin＇an 311300, Zhejiang, China) |
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| Abstract: | An efficient and reproducible protocol for large-scale micropropagation of Sasa argenteastriatus was developed with lateral buds as explants. A multiplication rate of 5.26 fold was obtained; on Murashige and Skoog＇s （MS） medium supplemented with 3.0 mg. L-1 benzyladenine （BA）. MS medium supplemented with 5.0 mg.1-1 indole-3-butyric acid （IBA） was most suitable for plantlet rooting. Rooted plantlets were hardened, ac- climatized and transferred to artificial mixture （peat ： vermiculite ： perlite = 1 ： 1 ： 1 ） with over 95% survival. |
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| Keywords: | botany Sasa argenteastriatus tissue culture |
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