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| 龙眼ISSR反应体系的建立和优化 | |
| 引用本文： | 曾黎辉,洪自同,许家辉,吴少华.龙眼ISSR反应体系的建立和优化[J].中国农学通报,2007,23(9):11-11. |
| 作者姓名： | 曾黎辉 洪自同 许家辉 吴少华 |
| 作者单位： | 1. 福建农林大学园艺学院,福州,350002;福建农林大学园艺植物遗传育种研究所,福州,350002 2. 福建农林大学园艺学院,福州,350002 3. 福建省农业科学院果树研究所,福州,350013 |
| 基金项目： | 福建省重大科技专项专题二“名特优水果种质资源保护及应用研究”（2004NZ02-2）,福建省科技计划重点项目“焦核龙眼ISSR分子标记研究”（2006N0003）. |
| 摘 要： | 对影响龙眼ISSR－PCR扩增反应的各个参数进行优化，建立适合龙眼的ISSR反应体系：PCR反应体积为20μl，其中模板DNA 25ng，引物0.2μmol/L，dNTP 100μmol/L，Taq DNA聚合酶0.5U，MgCl2 2.5mmol/L，10×PCR缓冲液2.0μl；扩增程序为94℃预变性5min；94℃变性1min，退火温度1min，72℃延伸90s，40个循环；72℃延伸7min |
| 关 键 词： | 稀土元素 稀土元素 植物 生物学作用 机制 |
| 修稿时间： | 2007-07-18 |
Establishment and Optimization of ISSR System in Dimocarpus Longan Lour. |
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| Zeng Lihui,Hong Zitong,Xu Jiahui,Wu Shaohua.Establishment and Optimization of ISSR System in Dimocarpus Longan Lour.[J].Chinese Agricultural Science Bulletin,2007,23(9):11-11. | |
| Authors: | Zeng Lihui Hong Zitong Xu Jiahui Wu Shaohua |
| Institution: | ;College of Hortictdture, Fuzhou 350002; lnstitute of Genetics and Breeding in Hortictdtural Plants, Fujian Agriculture and Forestry University, Fuzhou 350002; 3Fruit Science Institute, Fujian Academy of Agrieultural Science, Fuzhou 350013 |
| Abstract: | Factors affecting ISSR-PCR amplification of longan(Dimocarpus Longan Lour.) were optimized in this study and ISSR system for longan was established. Each 20uL amplification reaction consisted of 25ng DNA, 0.2 μ mol/L primer, 100 μmol/L each of dNTP, 0.5U Taq DNA polymerase, 2.5 mmol/L MgCl2 and 2.0 μl 10×PCR buffer; Amplification was under the following conditions: 5 min at 94℃ for 1 cycle, followed by 1 min at 94℃, 1 min at annealing temperature, and 90s at 72℃ for 40 cycles, and 7 min at 72℃ for a final extension. |
| Keywords: | Longan Dimocarpus Longan Lour ISSR Establishment of system Optimization |
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