| 速生杨愈伤组织染色体制片技术 |
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| 引用本文： | 王海燕.速生杨愈伤组织染色体制片技术[J].安徽农业科学,2008,36(25). |
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| 作者姓名： | 王海燕 |
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| 作者单位： | 新疆教育学院理学分院,新疆乌鲁木齐,830043 |
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| 基金项目： | 新疆生物资源基因工程重点实验室资助项目 |
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| 摘 要： | 目的]探索速生杨愈伤组织染色体制片的可行措施。方法]以中林46号速生杨愈伤组织为材料,取2 mm3左右生长旺盛的愈伤组织块,采用不同的预处理方法、解离及染色方法制备永久封片,对比不同方法的制片效果。结果]在5种预处理方法中,愈伤组织用4℃冰水预处理24 h或用0.05%秋水仙素在8~16℃条件下预处理6 h时,制片效果较好;在2种解离方法中,酸解和酶解愈伤组织效果相当,较理想;在3种染色方法中愈伤组织用吉姆萨染色2 h左右效果较好,改良苯酚品红染色2 h效果次之,醋酸洋红染色较浅,其显微摄影效果不理想。结论]用4℃冰水或0.05%秋水仙素预处理、用盐酸或含果胶酶、纤维素酶的甘露醇溶液解离、用吉姆萨染色时,速生杨愈伤组织染色体制片效果较好。
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| 关 键 词： | 速生杨 染色体 愈伤组织 |
Chromosome Preparation Technique of Callus of Fast-growing Poplar |
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| WANG Hai-yan.Chromosome Preparation Technique of Callus of Fast-growing Poplar[J].Journal of Anhui Agricultural Sciences,2008,36(25). |
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| Authors: | WANG Hai-yan |
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| Abstract: | Objective] The study was to explore the feasible measures on chromosome preparation of the callus of fast-growing poplar.Method] With callus of fast-growing poplar of Zhonglin 46 as material,the eugonic callus with about 2 mm3 were taken to prepare the permanent chromosome preparation with different methods of pretreatment,dissociation and staining and their the preparation effects were compared.Result] Among 5 pretreatment methods,the preparation effects were better when the callus was pretreated by ice water at 4 ℃ for 24 h or pretreated by 0.05% colchicine at 8-16 ℃ for 6 h.Among 2 dissociation methods,the effects of acidolysis and enzymatic hydrolysis on callus were equivalent,and they all were more perfect.Among 3 staining methods,the staining results of Giemsa was better when the callus was stained for about 2 h,the second was that stained by improved phenol fuchsin for about 2 h,and the staining color by aceto carmine was lighter,so its effect of photomicrography was non-ideal.Conclusion] The chromosome preparation effect of fast-growing poplar callus was better when callus was pretreated by ice water at 4 ℃ or by 0.05% colchicine,and dissociated by hydrochloride or mannitol solution that contained pectinase and cellulase and stained by Giemsa. |
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| Keywords: | Fast-growing poplar Chromosome Callus |
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