| 茶树根系HGMR基因克隆及表达分析 |
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| 引用本文： | 李远华,陆建良,范方媛,石玉涛.茶树根系HGMR基因克隆及表达分析[J].茶叶科学,2014,34(6):583-590. |
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| 作者姓名： | 李远华 陆建良 范方媛 石玉涛 |
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| 作者单位： | 1. 武夷学院茶与食品学院,福建 武夷山 354300; 2. 浙江大学茶叶研究所,浙江 杭州 310058 |
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| 基金项目： | 国家自然科学基金面上项目，福建省科技厅重点项目，国家级大学生创新创业训练计划项目 |
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| 摘 要： | 采用SSH技术分析了VA菌根处理后福鼎大白茶根系基因差异表达情况,获得了差异序列,序列比对显示,在上调表达序列中包含了3-羟基-3-甲戊二酸单酰辅酶A还原酶（3-hydroxy-3-methylglutaryl coenzyme A reductase,HMGR）。采用RACE技术获得了HGMR基因全长序列,HGMR基因长2 420 bp,具有1 773 bp开放阅读框（163rd~1935th）,编码590个氨基酸。分子生物信息学分析表明,HGMR蛋白分子量约63.5 kD,等电点为6.8,定位于线粒体膜或者内质网膜。研究还显示,HGMR在茶树叶片中表达强度存在明显的品种差异,同时,HGMR对生物性和非生物性胁迫均有明显响应。
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| 关 键 词： | 茶树 3-羟基-3-甲戊二酸单酰辅酶A还原酶 基因克隆 表达 |
| 收稿时间： | 2014-06-18 |
Gene Cloning and Expression Analysis of HMGR in Tea Plant Roots |
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| LI Yuanhua,LU Jianliang,FAN Fangyuan,SHI Yutao.Gene Cloning and Expression Analysis of HMGR in Tea Plant Roots[J].Journal of Tea Science,2014,34(6):583-590. |
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| Authors: | LI Yuanhua LU Jianliang FAN Fangyuan SHI Yutao |
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| Institution: | 1. College of Tea and Food Science, Wuyi University, Wuyishan 354300, China; 2. Zhejiang University Tea Research Institute, Hangzhou 310058, China |
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| Abstract: | By using SSH, the differences in gene expression of root system from Fuding Dabai tea infected by VA mycorrhiza were analyzed and diversity sequences were obtained. The sequence alignment showed that the mentioned sequence contain 3-hydroxy-3-methylglutaryl coenzyme A reductase named HMGR. HGMR full-length sequence was obtained by using RACE. The length of HGMR gene is 2 420 bp, with 1 773 bp ORF (163rd-1935th), and the sequence encoded 590 amino acids. Bioinformatics indicated that the HGMR protein’s molecular weight is about 63.5 kD, IEP is 6.8, which located in mitochondria or endoplasmic reticulum membrane. The study also showed expression degree of HGMR is distinct in different cultivars, while it responded obviously to biological and non-biological stress. |
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| Keywords: | tea plant 3-hydroxy-3-methylglutaryl coenzyme a reductase gene clone expression |
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