| 体细胞核移植法获得转人乳铁蛋白基因克隆山羊妊娠的研究(英文) |
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| 引用本文： | 刘凤军,张玉玲,杨自军,陈兴启,孙达权,王国华,张涌.体细胞核移植法获得转人乳铁蛋白基因克隆山羊妊娠的研究(英文)[J].农业科学与技术,2008,9(4):87-91. |
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| 作者姓名： | 刘凤军 张玉玲 杨自军 陈兴启 孙达权 王国华 张涌 |
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| 作者单位： | 河南科技大学动物科技学院;西北农林科技大学生物工程研究所 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，河南科技大学博士基金 |
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| 摘 要： | Objective] The aim of this study is to understand the effects of donor cell type,embryo stage,number and transfer position on the efficiency of goat transgenic clone.Method] Using somatic cell nuclear transfer technology,the single goat fetal fibroblasts(GFF)and mammary gland epithelial cells(GMGE)harboring human lactoferrin(hLF)gene were transferred to the enucleated oocyte.Reconstructed karyoplast-cytoplast couplets were fused,activated,and cultured in vitro.Embryos at 2-8 cell stage were transferred into oviduct of synchronized recipients,and blastocysts were transferred into uterine horn.Result] The pregnancy rate was similar between GFF and GMGE(oviduct transfer:26.47% vs.20.00%),and between oviduct transfer and uterine horn transfer(26.47% vs.25.00%)for GFF group;pregnancy rate in the group with the mean number of embryo transferred per recipient of 21.2 was significantly higher than in those the 5.93 group and 9.64 group(40.00% vs.26.67% and 21.43%).Conclusion] These results indicate that pregnancy rate of goat transgenic clone couldn’t be affected by donor cell type,embryo stage and transfer position but be done by the number of embryo transferred per recipient.In addition,the study also suggests the feasibility of making transgenic goat using GMGE as donor cells.
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| 关 键 词： | Somatic cell nuclear transfer Transgene Human lactoferrin Goat fetal fibroblasts Mammary gland epithelial cells |
Pregnancies Resulting from Transgenic Embryos with Human Lactoferrin Gene Produced by Somatic Cell Nuclear Transfer |
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| LIU Feng-jun,ZHANG Yu-ling,YANG Zi-jun,CHEN Xing-qi,SUN Da-quan,WANG Guo-hua,ZHANG Yong.Pregnancies Resulting from Transgenic Embryos with Human Lactoferrin Gene Produced by Somatic Cell Nuclear Transfer[J].Agricultural Science ＆ Technology,2008,9(4):87-91. |
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| Authors: | LIU Feng-jun ZHANG Yu-ling YANG Zi-jun CHEN Xing-qi SUN Da-quan WANG Guo-hua ZHANG Yong |
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| Institution: | 1.College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471003;2.Institute of Biotechnology,Northwest A & F University,Yangling 712100 |
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| Abstract: | Objective] The aim of this study is to understand the effects of donor cell type, embryo stage, number and transfer position on the efficiency of goat transgenic clone. Method] Using somatic cell nuclear transfer technology, the single goat fetal fibroblasts (GFF) and mammary gland epithelial cells (GMGE) harboring human lactoferrin (hLF) gene were transferred to the enucleated oocyte. Reconstructed karyoplast-cytoplast couplets were fused, activated, and cultured in vitro. Embryos at 2-8 cell stage were transferred into oviduct of synchronized recipients, and blastocysts were transferred into uterine horn. Result] The pregnancy rate was similar between GFF and GMGE (oviduct transfer:26.47% vs. 20.00%), and between oviduct transfer and uterine horn transfer (26.47% vs. 25.00%) for GFF group; pregnancy rate in the group with the mean number of embryo transferred per recipient of 21.2 was significantly higher than in those the 5.93 group and 9.64 group (40.00% vs. 26.67% and 21.43%). Conclusion] These results indicate that pregnancy rate of goat transgenic clone couldn't be affected by donor cell type, embryo stage and transfer position but be done by the number of embryo transferred per recipient. In addition, the study also suggests the feasibility of making transgenic goat using GMGE as donor cells. |
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| Keywords: | Somatic cell nuclear transfer Transgene Human lactoferrin Goat fetal fibroblasts Mammary gland epithelial cells |
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