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淡水黑鲷染色体核型分析
引用本文:孟庆磊,安丽,王成武,李娴,张志山,张龙岗,董学飒,杨玲,董俊,付佩胜.淡水黑鲷染色体核型分析[J].淡水渔业,2010,40(3).
作者姓名:孟庆磊  安丽  王成武  李娴  张志山  张龙岗  董学飒  杨玲  董俊  付佩胜
作者单位:山东省淡水水产研究所/山东省淡水水产遗传育种重点实验室,济南,250117
基金项目:山东省农业良种工程项目 
摘    要:以淡水黑鲷(Hephaestus fuliginosus)头肾组织为材料,采用腹腔注射植物血细胞凝集素(PHA)、秋水仙素和空气干燥法制备染色体标本,进行染色体核型分析。结果显示,淡水黑鲷染色体为48条,核型公式为2n=48=4m+44t,NF=52;未发现随体、次级缢痕及异形性染色体;其核型符合典型的高位类群鱼类核型特征。

关 键 词:淡水黑鲷(Hephaestus  fuliginosus)  染色体  核型

Karyotype analysis in the black bream(Hephaestus fuliginosus)
MENG Qing-lei,AN Li,WANG Cheng-wu,LI Xian,ZHANG Zhi-shan,ZHANG Long-gang,DONG Xue-sa,YANG Ling,DONG Jun,FU Pei-sheng.Karyotype analysis in the black bream(Hephaestus fuliginosus)[J].Freshwater Fisheries,2010,40(3).
Authors:MENG Qing-lei  AN Li  WANG Cheng-wu  LI Xian  ZHANG Zhi-shan  ZHANG Long-gang  DONG Xue-sa  YANG Ling  DONG Jun  FU Pei-sheng
Institution:MENG Qing-lei,AN Li,WANG Cheng-wu,LI Xian,ZHANG Zhi-shan,ZHANG Long-gang,DONG Xue-sa,YANG Ling,DONG Jun,FU Pei-sheng(Shandong Provincial Key Lab for Genetics & Breeding of Freshwater Fisheries,Freshwater Fisheries Research Institute of Shandong Province,Jinan 250117)
Abstract:Karyotype of the Black bream Hephaestus fuliginosus was studied with chromosome samples prepared from head kidney by PHA and colchicine injection and air-dryed method.The result showed that there were 48 chromosomes in H.fuliginosus and its karyotype formula was 2n=48=4m+44t,NF=52.There was no visible evidence of heterotypical chromosome,satellites or secondary constrictions.The karyotype of H.fuliginosus is in accordance with the higher group of fish evolutionary taxonomy.
Keywords:black bream(Hephaestus fuliginosus)  chromosome  karyotype  
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