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河北省昆虫病原线虫资源调查
引用本文:陈书龙,李秀花,史均环.河北省昆虫病原线虫资源调查[J].中国生物防治,2006,22(1):40-44.
作者姓名:陈书龙  李秀花  史均环
作者单位:1. 河北省农林科学院植物保护研究所,保定,071000
2. 河北省沧县植保站
基金项目:河北省自然科学基金;国家留学回国人员科研启动基金
摘    要:采用大腊螟诱捕法,对采自河北省的1975个土样进行昆虫病原线虫分离。共分离出斯氏属线虫17个种群,异小杆属线虫49个种群,土壤带线虫率为3.34%。在斯氏属线虫中,其中格氏线虫有2个种群,新种A有一个种群,新种B有14个种群;而49个异小杆线虫种群均为嗜菌异小杆线虫。在河北南部邯郸及邢台地区,以斯氏线虫为主,而在中北部地区则以异小杆线虫为主。同时明确土样中的带线虫率与土壤植被以及土壤质地密切相关。未耕地以及以果树、蔬菜和大田作物为植被的土壤带线虫率分别为6.59%、5.13%、3.15%和2.9%;沙土、沙壤土、壤土以及粘土的带线虫率分别为8.57%、3.17%、3.36%和0。

关 键 词:生物防治  昆虫病原线虫  异小杆线虫  斯氏线虫  调查
文章编号:1005-9261(2006)01-0044-05
收稿时间:2005-03-14
修稿时间:2005年3月14日

Occurrence of Entomopathogenic Nematodes in Hebei Province
CHEN Shu-long,LI Xiu-hua,SHI Jun-huan.Occurrence of Entomopathogenic Nematodes in Hebei Province[J].Chinese Journal of Biological Control,2006,22(1):40-44.
Authors:CHEN Shu-long  LI Xiu-hua  SHI Jun-huan
Institution:Institute of Plant Protection, Hebei Academy of Agricultural arid Forestry Sciences, Baoding 071000, China
Abstract:1975 soil samples were collected from different areas in Hebei Province and the entomopathogenic nematodes(EPN) were extracted with Galleria mellonella trap method.A total of 66 populations were isolated from soil samples,among which 2 populations are Steinernema glaseri, 49 populations are Heterorhabditis bacteriophora. One population(G6) isolated from Gu'an county is a new steinernematide species that can be distinguished from all known species by morphology,morphometrics and ITS-rDNA sequence analysis,and the others were confirmed to be another new steinernematide species.It was determined that occurrence of EPN was closely related to plant species and soil types.The prevalence of EPN in uncultivated soil,orchard,vegetable fields and crop fields were 6.59%,5.13%,3.15% and 2.90%,respectively;and in sandy,sandy loam,loam and clay soils,8.57%,3.17%,3.36% and 0,respectively.
Keywords:biological control  entomopathogenic nematodes  Heterorhabditis  Steinernema  survey
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