| 玫烟色棒束孢FZ-01鉴定及对烟粉虱的防治潜力评估 |
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| 引用本文： | 郑宇,丁雪玲,姚凤銮,卢学松,卓辉,何玉仙.玫烟色棒束孢FZ-01鉴定及对烟粉虱的防治潜力评估[J].中国生物防治学报,2019,35(6):876-883. |
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| 作者姓名： | 郑宇 丁雪玲 姚凤銮 卢学松 卓辉 何玉仙 |
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| 作者单位： | 1. 福建省农业科学院植物保护研究所/农业部福州作物有害生物科学观测实验站/福建省作物有害生物监测与治理重点实验室, 福州 350013;2. 闽台作物有害生物生态防控国家重点实验室/福建农林大学, 福州 350002;3. 福建中医药大学药学院, 福州 350108 |
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| 基金项目： | 福建省公益类科研院所专项（2015R1024-4，2017R1025-1）；福建省科技重大专项（2017NZ0003-1） |
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| 摘 要： | 为了明确从罹病烟粉虱Bemisia tabaci上分离的虫生真菌（菌株FZ-01）的分类归属及其生物防治的潜力，采用形态学特征观察和rDNA-ITS序列分析方法对该菌株进行鉴定，并对其生物防治潜力进行评估。结果发现：菌株FZ-01为玫烟色棒束孢Isaria fumosorosea；该菌株在马铃薯PDA培养基上生长良好；适应温度和酸碱度范围较广，可耐高温（35℃）和酸碱（pH4～10）；孢子萌发速率快（5 h）且萌发率高（>90%）；菌株致病力测定表明分生孢子浓度为1.0×107 cfu/mL时，对烟粉虱（MEAM1）成虫致死中时间（LT50）仅为2.75 d，接菌4 d时致死中浓度（LC50）仅为2.69×104 cfu/mL。综上结果表明，玫烟色棒束孢FZ-01对烟粉虱具有十分优良的生防潜力。
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| 关 键 词： | 虫生真菌 玫烟色棒束孢 烟粉虱 生防潜力 |
| 收稿时间： | 2019-02-26 |
Identification of Isaria fumosorosea FZ-01 and Evaluation of Its Bio-control Potential against Bemisia tabaci |
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| ZHENG Yu,DING Xueling,YAO Fengluan,LU Xuesong,ZUO Hui,HE Yuxian.Identification of Isaria fumosorosea FZ-01 and Evaluation of Its Bio-control Potential against Bemisia tabaci[J].Chinese Journal of Biological Control,2019,35(6):876-883. |
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| Authors: | ZHENG Yu DING Xueling YAO Fengluan LU Xuesong ZUO Hui HE Yuxian |
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| Abstract: | A strain of entomopathogenic fungi (FZ-01) isolated from Bemisia tabaci was identified morphologically and using molecular method employing rDNA-ITS gene sequences. The biocontrol potential of the strain was also evaluated. The strain FZ-01 was identified as Isaria fumosorosea, growing well on the potato medium (PDA), showing wide adaptability to temperatures (above 35℃) and pH values (pH 4-10), and with fast spore germination speed (5 h) and high spore germination rate (>90%). Under laboratory conditions, the virulence test showed that the strain at conidium concentration of 1.0×107cfu/mL was characterized by the medium lethal time (LT50) of 2.75 d and the medium lethal concentration (LC50) of 2.69×104cfu/mL at 4 d after inoculation. The results indicate that the strain FZ-01 possesses promising biocontrol potential against B. tabaci. |
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| Keywords: | entomopathogenic fungi Isaria fumosorosea Bemisia tabaci bio-control potential |
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