| 水稻细菌性条斑病生防菌株筛选和田间防治效果 |
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| 引用本文： | 王星,杨俊,曹卫华,魏兰芳,姬广海.水稻细菌性条斑病生防菌株筛选和田间防治效果[J].中国生物防治学报,2019,35(4):648-654. |
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| 作者姓名： | 王星 杨俊 曹卫华 魏兰芳 姬广海 |
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| 作者单位： | 1. 云南农业大学/农业生物多样性与病虫害控制教育部重点实验室, 昆明 650201;2. 云南农业大学农科基础实验教学中心, 昆明 650201 |
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| 基金项目： | 云南省科技厅农业基础研究联合专项（2017FG001-005）；国家重点研发计划（2018YFD0200308，2017YFC1702502，2018YFD0200703）；云南省科技厅重点研发（农业领域）项目（2018BB016）；国家自然科学基金（31460458） |
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| 摘 要： | 为筛选防治水稻细菌性条斑病的生防菌株，通过平板对峙试验、温室及田间试验，测定了4个菌株对水稻促生和防治细菌性条斑病的效果。通过平板对峙试验明确了4株生防菌株抑菌活性；温室试验中，除生防菌LH外，其他3株生防菌的发酵液处理后的水稻幼苗的株高、根系、鲜重均显著高于对照。接种结果显示接种病原菌前喷施生防菌防效最好。生防菌L1在3个不同试验田中防效均达到55%以上，同时增产效果达到10%以上，显著高于其他处理。研究表明，生防菌L1不仅可以用于水稻细菌性条斑病的生物防治，而且可以促进水稻生长，具有较好的生产应用潜力。
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| 关 键 词： | 水稻细菌性条斑病 生防细菌 促生 生物防治 |
| 收稿时间： | 2018-10-10 |
Screening of Biocontrol Bacteria on Rice Bacteria Leaf Streak and Determination of Field Control Efficacy |
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| WANG Xing,YANG Jun,CAO Weihua,WEI Lanfang,JI Guanghai.Screening of Biocontrol Bacteria on Rice Bacteria Leaf Streak and Determination of Field Control Efficacy[J].Chinese Journal of Biological Control,2019,35(4):648-654. |
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| Authors: | WANG Xing YANG Jun CAO Weihua WEI Lanfang JI Guanghai |
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| Institution: | 1. Key Laboratory of Agriculture Biodiversity for Plant Disease Management, the Ministry of Education/Yunnan Agricultural University, Kunming 650201, China;2. Agricultural Foundation Experiment Teaching Center, Yunnan Agricultural University, Kunming 650201, China |
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| Abstract: | In order to screen better biocontrol bacteria against rice bacteria leaf streak (BLS) caused by Xanthomonas oryzae pv.oryzicola, the biocontrol efficacy of four biocontrol bacteria strains on rice bacteria leaf streak were evaluated by plate confrontation test, greenhouse and field experiments. In the plate confrontation test, the antibacterial activities of the four biocontrol bacteria strains were determined. Greenhouse experiment showed that except for biocontrol strain LH, the plant height, root length and fresh weight of the rice seedlings treated with the other three biocontrol bacteria fermentation broths were significantly higher than those of the control. Inoculation test indicated that application of biocontrol bacteria before inoculation of pathogenic X. oryzae had the best control effect. Field trials demonstrated that the biocontrol strain L1 achieved more than 55% control efficacy in three different fields, and the yield increase rate was more than 10%, which was significantly higher than other treatments. This study shows that the biocontrol strain L1 can not only be used for the biological control of the BLS disease, but also promote rice growth, thus it has a good potential application. |
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| Keywords: | rice bacteria leaf streak biocontrol bacteria plant growth promotion biological control |
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