The effect of colchicine on triticale anther-derived plants: Microspore pre-treatment and haploid-plant treatment using a hydroponic recovery system |
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| Authors: | Ahmad Arzani Norman L Darvey |
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| Institution: | (1) Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan, 84156, Iran;(2) University of Sydney, Plant Breeding Institute, Cobbitty, Private Bag 11, Camden, NSW, 2570, Australia |
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| Abstract: | In cereals, chromosome doubling of microspore-derived haploid plants is a critical step in producing doubled haploid plants.
This investigation was undertaken to study the effect of incorporation of colchicine in the induction medium for anther culture,
and the effect of colchicine on anther culture-derived plants of triticale grown under controlled greenhouse conditions. In
the latter case, chromosome doubling of adult sterile plants derived from anther culture of fourteen triticale populations
was attempted, where androgenetic plants with non-dehiscent anthers were cloned and subjected to the colchicine treatment,
and then grown with the aid of hydroponics. The hydroponic system provided optimal conditions for recovery of the affected
haploids from the toxic effects of colchicine treatment and all colchicine-treated plants survived. A topcross-F1 (TC1F1) population with timopheevii cytoplasm produced the highest percentage of plants with seed-set either due to chromosome doubling
by colchicine (98%) or spontaneous doubling of chromosome number (15%). Colchicine-treated anthers performed inferior than
control in both induction and regeneration phases. One of the key observation of this study was the reversal from reproductive
stage back to the vegetative stage which in turn enabled further cloning of haploid plants under hydroponic conditions once
they were identified as sterile. The one hundred percent survival rate of in vitro-derived plants, 100% survival rate of colchicine treated haploid plants and the high chromosome doubling success rate (X
= 82.3) observed in this study imply that a temperature-controlled greenhouse with an hydroponic system provides an efficient
environment for inducing chromosome doubling of haploid plants in cereals.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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| Keywords: | anther culture chromosome doubling colchicine haploid hydroponics Triticale X triticosecale |
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