| 基于RAPD和EST-SSR标记的秀珍菇菌株聚类分析 |
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| 引用本文： | 忻雅,阮松林,王世恒,马华升,王伟科,王淑珍.基于RAPD和EST-SSR标记的秀珍菇菌株聚类分析[J].食用菌学报,2008,15(4). |
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| 作者姓名： | 忻雅 阮松林 王世恒 马华升 王伟科 王淑珍 |
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| 作者单位： | 杭州市农业科学研究院,浙江杭州,310024 |
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| 基金项目： | 杭州市珍稀食用菌科技创新服务平台(编号：20052212Y10)的部分研究内容 |
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| 摘 要： | 本研究利用RAPD标记和EST-SSR标记对10个不同来源的秀珍菇菌株进行聚类分析。200条RAPD引物中127条有扩增产物，引物可用率为63．5％。从NCBI数据库中下载秀珍菇及相近侧耳属食用菌EST序列2167条，聚类比对后得到全长为713．541kb的非冗余EST1442条，搜寻后得到的29个EST-SSR全部设计引物，其中26对引物（89．7％）显示多态性。根据17条RAPD核心引物和10对EST-SSR核心引物的扩增结果，对10个秀珍菇菌株进行了RAPD标记、EST-SSR标记及二者相结合的聚类分析。3种分析结果相近，且与菌丝、子实体生长特性分析结果相统一——10个供试菌株区分为5组：1～4号菌株为一组，6号和7号菌株为一组，8号和9号菌株为一组，5号和10号菌株各自为一组。
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| 关 键 词： | 秀珍菇 RAPD标记 EST-SSR标记 聚类分析 |
Cluster Analysis of Ten Pleurotus geesteranus Strains Based on Random Amplification of Polymorphic DNA (RAPD) and Expressed Sequence Tag Single Sequence Repeats (ESTSSR) Markers |
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| XIN Ya,RUAN Songlin,WANG Shiheng,MA Huasheng,WANG Weike,WANG Shuzhen.Cluster Analysis of Ten Pleurotus geesteranus Strains Based on Random Amplification of Polymorphic DNA (RAPD) and Expressed Sequence Tag Single Sequence Repeats (ESTSSR) Markers[J].Acta Edulis Fungi,2008,15(4). |
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| Authors: | XIN Ya RUAN Songlin WANG Shiheng MA Huasheng WANG Weike WANG Shuzhen |
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| Abstract: | Amplification products were obtained from ten strains of Pleurotus geesteranus using 127 (63.5%) of 200 RAPD primers tested.A total of 2167 Expressed Sequence Tags (ESTs) from P.geesteranus and related Pleurotus species were downloaded from the NCBI database and,after clustering,1442 non- redundant ESTs with a total length of approximately 713,541 bp were obtained.Twenty-nine Expressed Sequence Tag- Single Sequence Repeats (EST-SSRs) were selected from these non-redundant ESTs and used to design EST-SSR pri... |
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| Keywords: | Pleurotus geesteranus RAPD marker EST-SSR marker cluster analysis |
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