Usefulness of AFLP markers to estimate varietal homogeneity of rapeseed inbred line varieties in the context of plant registration and protection |
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| Authors: | Vincent Lombard Benoit Tireau Françoise Blouet David Zhang Claire P Baril |
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| Institution: | 1. GEVES, Groupe d'Etude et de Contr?le des Variétés et des Semences, domaine du Magneraud, BP 52, F-17700, Surgères, France
2. GEVES, Groupe d'Etude et de Contr?le des Variétés et des Semences, La Minière, F-78285, Guyancourt, France
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| Abstract: | The ability of AFLP markers to assess the uniformity of rapeseed inbred line varieties was investigated in the context of
plant registration. Three candidate varieties were chosen on a scale of morphological heterogeneity in the field. Thirty rows
per variety were characterized with 4 primer combinations (173 markers). Principal component analysis and distribution of
pairwise Rogers' distances between rows revealed exactly the same off types as those observed in the fields. The Nei's genetic
diversity computed from the binary data matrix revealed the same diversity level as the one observed with morphological traits:
the Nei's diversity value for the most homogeneous variety, a microspore-derived doubled haploid line, was 0.0011, whereas
the value for the most heterogeneous variety was 0.0706. The similarity between the results with AFLP markers and morphological
traits showed that AFLP markers associated with adapted data analysis tools (PCA, Nei's diversity index, genetic distance
distribution) are very useful to assess the uniformity of candidate varieties in the context of plant registration.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | AFLP inbred lines plant registration rapeseed varietalhomogeneity |
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