| 24-表油菜素内酯对低氧胁迫下黄瓜幼苗根系抗氧化系统的影响 |
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| 引用本文： | 康云艳,郭世荣,李娟,段九菊.24-表油菜素内酯对低氧胁迫下黄瓜幼苗根系抗氧化系统的影响[J].中国农业科学,2008,41(1):153-161. |
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| 作者姓名： | 康云艳 郭世荣 李娟 段九菊 |
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| 作者单位： | 南京农业大学园艺学院,南京,210095 |
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| 摘 要： | 【目的】探讨外源EBR（24-表油菜素内酯）对低氧胁迫下黄瓜（Cucumis sativus L.）根系的生长和抗氧化系统的影响。【方法】采用营养液水培法，以抗低氧能力较强的黄瓜品种绿霸春4号和抗低氧能力较弱的品种中农8号为材料，研究根际低氧胁迫下外源施用EBR对黄瓜根系生长、ROS及抗氧化酶活性的影响。【结果】在低氧胁迫下，EBR处理显著促进了低氧胁迫下两品种黄瓜幼苗根系生长，提高了SOD、APX、GR活性及AsA、GSH含量，降低了O 、H2O2及MDA含量。【结论】外源EBR处理通过促进低氧胁迫下黄瓜幼苗根系中抗氧化酶活性和抗氧化剂含量的提高，降低ROS含量，增强植株抗低氧胁迫的能力。
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| 关 键 词： | 黄瓜 24-表油菜素内酯 幼苗 低氧胁迫 抗氧化系统 膜脂过氧化 |
| 收稿时间： | 2006-09-07 |
| 修稿时间： | 2006-11-28 |
Effects of 24-Epibrassinolide on Antioxidant System in Cucumber Seedlings Roots Under Hypoxia Stress |
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| KANG Yun-yan,GUO Shi-rong,LI Juan,DUAN Jiu-ju.Effects of 24-Epibrassinolide on Antioxidant System in Cucumber Seedlings Roots Under Hypoxia Stress[J].Scientia Agricultura Sinica,2008,41(1):153-161. |
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| Authors: | KANG Yun-yan GUO Shi-rong LI Juan DUAN Jiu-ju |
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| Abstract: | Effects of 24-Epibrassinolide on Antioxidant System in Cucumber Seedlings Roots under Hypoxia Stress
KANG Yun-yan , GUO Shi-rong , Duan Jiu-ju
(College of Horticulture, Nanjing Agriculture University, Nanjing 210095)
Abstract: Objective] The aim of our work was to study the effects of exogenous 24-epibrassinolide (EBR) on ROS, activities of antioxidant enzymes and antioxidants and their time courses in cucumber (Cucumis sativus L.) seedling roots under hypoxia stress. Method] Seedlings of a hypoxia-resistant cultivar, Lübachun No.4, and a hypoxia-sensitive cultivar, Zhongnong No.8, were subjected to nutrient solution hypoxia stress treatments. The effects of EBR on the growth, ROS, antioxidant enzymes and antioxidants in seedlings roots were investigated. Results] Under hypoxia stress, O2?– level and lipid peroxidation were significantly increased in the roots upon exposure to hypoxia stress, which were inhibited by exogenous EBR application. EBR treatment significantly increased seedlings growth, SOD、APX、GR activities and contents of AsA、GSH under hypoxia stress. Conclusion] From the results obtained in this work it can be conclude that oxidative damage on seedling roots by hypoxia stress can be considerably alleviated, which elevated the tolerance of plants. |
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| Keywords: | 24-Epibrassinolide Cucumber seedlings Hypoxia stress Antioxidative system Lipid peroxidation |
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