| 几种外部因子对春石斛组培生根的影响 |
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| 引用本文： | 潘虹虹,孙丹,廉美兰,朴炫春.几种外部因子对春石斛组培生根的影响[J].安徽农业科学,2008,36(29). |
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| 作者姓名： | 潘虹虹 孙丹 廉美兰 朴炫春 |
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| 作者单位： | 延边大学农学院园艺系,吉林龙井,133400;延边大学农学院园艺系,吉林龙井,133400;延边大学农学院园艺系,吉林龙井,133400;延边大学农学院园艺系,吉林龙井,133400 |
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| 基金项目： | 国家自然科学基金
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| 摘 要： | 目的]为春石斛种苗的规模化生产提供理论依据。方法]以春石斛组培增殖的不定芽为外植体,研究MS培养基浓度、IBA浓度、活性炭浓度及天然物质等外部因子对春石斛试管苗生根的影响。结果]MS培养基浓度对试管苗生根影响较大,试管苗在1/2 MS培养基中生根效果较好,生根率达95%,根多且长。在1/2 MS培养基中加入0.5 mg/LIBA和0.5 mg/L活性炭对试管苗生根及生长有明显促进作用。在培养基中添加天然物质对试管苗生根有促进作用,其中添加100 g/L椰子汁时生根效果最佳,生根率可达97%。结论]当培养基配方为1/2 MS+0.5 mg/LIBA+0.5 mg/L活性炭+100 g/L椰子汁时,春石斛试管苗生根及生长效果较好。
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| 关 键 词： | 春石斛 培养基浓度 活性炭 天然物质 |
Effect of Several External Factors on Rooting of Tissue Culture Seedling of Dendrobium nobile |
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| Abstract: | Objective] The study was to provide the theoretical basis for scale production of Dendrobium nobile seedlings.Method] With adventitious buds proliferated through tissue culture of D.nobile as explants,the effects of several external factors such as the concn.of MS medium,IBA,activated carbon and natural substance etc on rooting of tube seedlings of D.nobile were studied.Result] The effect of MS medium concn.on rooting of tube seedling was bigger,and the rooting effect of tube seedling in 1/2 MS medium was better,the rooting rate reached 95%,and the roots were more and long.It had obvious promoting effect on rooting and growth of tube seedling when IBA at 0.5 mg/L and activated carbon at 0.5 mg/L were added into 1/2 MS medium.It had promoting effect on rooting of tube seedling when natural substance was added into the medium,and the rooting effect was best when 100 g/L coconut juice was added into the medium,with rooting rate up to 97%.Conclusion]The effect of rooting and growth of tube seedling of D.nobile were better when the formula of medium was 1/2 MS +0.5 mg/L IBA +0.5 mg/L activated carbon+100 g/L coconut juice. |
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| Keywords: | Dendrobium nobile Medium concn Activated carbon Natural substances |
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