| 苦瓜果肉蛋白的纯化及抑制作用研究 |
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| 引用本文： | 孔立华,朱新产.苦瓜果肉蛋白的纯化及抑制作用研究[J].江西农业学报,2009,21(7):134-136. |
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| 作者姓名： | 孔立华 朱新产 |
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| 作者单位： | 青岛农业大学,生命科学学院,山东,青岛,266109 |
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| 摘 要： | 以新鲜苦瓜果肉为材料,用沉淀和层析技术方法制备得到纯化苦瓜蛋白,SDS-PAGE电泳显示为单一条带。用不同浓度的纯化苦瓜蛋白对豆类种子(绿豆、红豆、黄豆)、谷类种子(小麦、玉米、水稻)进行抑制试验,结果表明:纯化苦瓜蛋白对种子萌发有抑制作用,随其浓度的升高,种子淀粉酶活性和可溶性糖含量降低,蛋白质含量升高。纯化苦瓜蛋白浓度为2.5 mg/mL时对各种子生化指标的影响最大。提示纯化苦瓜蛋白是有效的抑制剂药用成分。
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| 关 键 词： | 苦瓜蛋白 抑制作用 淀粉酶活力 蛋白质含量 可溶性糖含量 |
Study on Inhibition of Purified Protein from Momordica charantia |
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| KONG Li-hua,ZHU Xin-chan.Study on Inhibition of Purified Protein from Momordica charantia[J].Acta Agriculturae Jiangxi,2009,21(7):134-136. |
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| Authors: | KONG Li-hua ZHU Xin-chan |
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| Institution: | College of Life Sciences;Qingdao Agricultural University;Qingdao 266109;China |
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| Abstract: | The purified protein was prepared from the fresh fruit of Momordica charantia by precipitation and chromatography techniques,SDS-PAGE electrophoresis showed a single band.The inhibitive effects of different concentrations of above-mentioned purified protein to the seeds of legume(mung bean,jumble beads and soybean) and cereal(wheat,corn and rice) were tested.The results showed that the purified protein had an inhibition on the germination of above seeds,the seed amylase activity and soluble sugar content de... |
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| Keywords: | Protein from Momordica charantia Inhibition Amylase activity Content of protein Content of soluble-sugar |
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