Spawning induction and hormonal levels during final oocyte maturation in the silver perch (Bidyanus bidyanus) |
| |
| Institution: | 1. Department of Animal Sciences, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University, P.O. Box 12, Rehovot 76100, Israel;2. Aquaculture Research Station-DOR, M.P Hof Hacarmel 30820, Israel;1. Department of Integrative Biology, University of Guelph, ON N1G 2W1, Canada;2. Great Lakes Laboratory for Fisheries and Aquatic Sciences, Fisheries and Oceans Canada, 867 Lakeshore Rd, Burlington, ON L7R 4A6, Canada;3. Department of Biological Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON M1C 1A4, Canada;1. Centro de Ciências do Mar do Algarve, Universidade do Algarve. Campus de Gambelas, 8005-139 Faro, Portugal;2. Instituto Português do Mar e da Atmosfera - IPMA, Av. Brasília s/n, 1449-006 Lisboa, Portugal;3. Department of Biological and Environmental Sciences, College of Arts and Sciences, Qatar University, PO Box 2713 Doha, Qatar;4. Marine and Environmental Sciences Centre, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal;5. Centre for Marine and Environmental Research (CIMA), University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal;1. Department of Natural Products, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia;2. Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Assiut Branch, Assiut 71524, Egypt;3. Natural Products Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia;4. Suez Canal University Hospital, Suez Canal University, Ismailia 41522, Egypt;5. Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt;6. Department of Clinical Biochemistry, Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi Arabia;7. Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt;1. Programa de Pós-Graduação em Zoologia, UFPA-MPEG, Avenida Perimetral 1901, CEP 66077-830 Belém, PA, Brazil;2. Coordenação de Ciências da Terra e Ecologia, Museu Paraense Emilio Goeldi, Avenida Perimetral 1901, CEP 66077-830 Belém, PA, Brazil;1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China;2. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China;3. University of Chinese Academy of Sciences, Beijing, China |
| |
| Abstract: | The silver perch (Bidyanus bidyanus Mitchell) (Teraponidae), is a native Australian freshwater fish that, due to its high potential for aquaculture, was introduced to Israeli fish farming. The objective of this study was to find an optimal method for inducing spawning of silver perch. The agents tested for this purpose were: human chorionic gonadotropin (hCG; 150 or 200 IU/kg BW); salmon gonadotropin releasing hormone analogue (sGnRHa at 10, 20, 30, or 40 μg/kg BW); mammalian GnRH analogue (mGnRHa at 30 μg/kg) and the combination of sGnRHa at 20 μg/kg and domperidone at 5 mg/kg BW. Based on spawning success and relative fecundity, sGnRHa at the dose of 30 μg/kg was found to be more efficient than hCG, mGnRHa or sGnRHa with domperidone. Since domperidone does not improve the GnRHa effect on final oocyte maturation (FOM) and spawning, it is suggested that the dopaminergic inhibition during the stages of FOM in the silver perch is weak. Therefore, the use of GnRHa alone is sufficient to induce spawning in this fish. Immunoreactive gonadotropin (IR-GtH) and estradiol levels increased after a single injection of sGnRHa, and peaked after 24 h. Plasma levels of 17α,20β-dihydroxy-4-pregnen-3-one (17,20-P) also increased significantly 24 h after the injection of mGnRHa, 12 h before spawning, suggesting that 17,20-P is the maturation-inducing steroid in silver perch. In order to reveal whether the heterologous gonadotropin may elicit an immunological reaction, silver perch was subjected to prolonged treatment with hCG. This treatment resulted in no detectable titer of antibodies against the mammalian gonadotropin. In conclusion, although hCG has no deleterious effects in this fish, and is the more commonly used for spawning induction, sGnRHa at 30 μg/kg is the recommended treatment for spawning induction of female silver perch under the conditions prevailing in Israeli aquaculture. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
