| 基于对瓦布贝母核苷类成分贡献率的锌硼钼最优配施浓度 |
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| 引用本文： | 陈雨,张亚琴,邓秋林,杨正明,雷飞益,付羽萍,刘震东,陈兴福.基于对瓦布贝母核苷类成分贡献率的锌硼钼最优配施浓度[J].植物营养与肥料学报,2019,25(8):1401-1412. |
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| 作者姓名： | 陈雨 张亚琴 邓秋林 杨正明 雷飞益 付羽萍 刘震东 陈兴福 |
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| 作者单位： | 1.四川农业大学农学院/农业部西南作物生理生态与耕作重点实验室，成都 611130 |
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| 基金项目： | 四川省“十三五育种攻关”项目（2016NYZ0036-3-2）。 |
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| 摘 要： | 【目的】核苷类成分为贝母的主要活性物质，本研究分析了锌硼钼配施对瓦布贝母核苷类成分含量的影响，以优化瓦布贝母最佳微肥施用配方。【方法】以鳞茎大小为12 mm的四年生瓦布贝母为种源，在四川川贝母标准化生产基地进行田间小区试验。设置锌肥、硼肥、钼肥的三因素五水平二次正交旋转组合设计。在瓦布贝母现蕾期、初花期、盛花期、幼果期进行叶面喷施，喷施量分别为各微量元素总量的20%、30%、30%、20%。五年生瓦布贝母倒苗后采收鳞茎，测定了干鳞茎中10种核苷含量。采用最小二乘法、灰色关联度和DTOPSIS法计算每种或几种微量元素含量及对核苷含量的贡献率，提出优化施肥方案。【结果】根据贡献率分析表明，锌肥对尿嘧啶核苷、胸腺嘧啶和肌苷贡献率最大，硼肥对胞苷和鸟嘌呤核苷贡献率最大，钼肥对尿嘧啶和胸苷贡献率最大。单因素效应分析表明，锌肥和硼肥对胞苷有显著影响，钼肥对胸苷有显著影响。互作效应分析表明，锌–硼互作对尿嘧啶产生协同作用，对胸腺嘧啶、尿嘧啶核苷和胸苷产生拮抗作用；锌–钼互作对尿嘧啶、尿嘧啶核苷和肌苷产生协同作用；硼–钼互作对胞苷、胸苷、鸟嘌呤核苷和尿嘧啶核苷产生拮抗作用。灰色关联度和DTOPSIS法分析得出核苷含量综合最优处理。【结论】确定最优的施肥范围:锌肥为8.05～11.85 kg/hm2；硼肥为11.70～16.47 kg/hm2；钼肥为0.36～0.51 kg/hm2。通过灰色关联度和DTOPSIS法进行分析得出，当使用锌肥15.71 kg/hm2、硼肥5.53 kg/hm2、钼肥为0.65 kg/hm2时瓦布贝母10种核苷的含量综合最优。
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| 关 键 词： | 瓦布贝母 锌 硼 钼 核苷 二次正交旋转 |
| 收稿时间： | 2018-05-31 |
Optimum combining concentration of zinc,boron and molybdenum foliar application for high content of nucleosides in Fritillaria unibracteata var. wabuensis |
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| CHEN Yu,ZHANG Ya-qin,DENG Qiu-lin,YANG Zheng-ming,LEI Fei-yi,FU Yu-ping,LIU Zhen-dong,CHEN Xing-fu.Optimum combining concentration of zinc,boron and molybdenum foliar application for high content of nucleosides in Fritillaria unibracteata var. wabuensis[J].Plant Nutrition and Fertilizer Science,2019,25(8):1401-1412. |
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| Authors: | CHEN Yu ZHANG Ya-qin DENG Qiu-lin YANG Zheng-ming LEI Fei-yi FU Yu-ping LIU Zhen-dong CHEN Xing-fu |
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| Institution: | 1.College of Agronomy, Sichuan Agricultural University/Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, Chengdu 611130, China |
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| Abstract: | 【Objectives】Nucleoside component is one of the main active substances of Fritillaria. The contribution rate of Zn, B and Mo application on the content of nucleosides in Fritillaria was studied for the optimization of their application formula of Fritillaria unibracteata var. wabuensis.【Methods】A plot experiment was conducted at the standardized production base of Sichuan Fritillaria. The F. unibracteata var. wabuensis bulbs of four-year-old and 12 mm in size were used as materials. A five-level quadratic orthogonal rotation method was used for the treatment design of the experiment. The 20%, 30%, 30% and 20% of the total Zn, B and Mo fertilizers were foliar applied at the budding, early flowering, full flowering and young fruit stages of Fritillaria, respectively. The Fritillaria bulbs were harvested from the experimental plots, and they were used to determine the content of 10 nucleosides.【Results】The least square method, gray correlation degree and DTOPSIS method were used to calculate the contribution rates of trace elements and nucleosides, the influence of the single factors and interaction of two factors on the nucleoside components to obtain the optimal fertilization recommendation.【Results】By contribution rate analysis, zinc fertilizer contributed the most to uridine, thymidine and inosine, boron fertilizer contributed the most to cytidine and guanosine, and molybdenum contributed the most to uracil and thymidine. Single factor analysis showed that zinc fertilizer and boron fertilizer had significant effects on cytidine, and molybdenum fertilizer had significant effect on thymidine. Interaction analysis showed that zinc-boron interaction synergistically exerted uracil, but antagonized thymine, uridine and thymidine; zinc-molybdenum interaction synergized with uracil, uridine and inosine; boron-molybdenum interaction antagonized cytidine, thymidine, guanosine and uridine. The grey correlation degree and DTOPSIS method analysis showed the comprehensive optimal treatment.【Conclusions】The optimal fertilization was determined as zinc fertilizer 8.05–11.85 kg/hm2; boron fertilizer 11.70–16.47 kg/hm2; and molybdenum fertilizer 0.36–0.51 kg/hm2. By using grey correlation and DTOPSIS method, when the zinc fertilizer was 15.71 kg/hm2, boron fertilizer 5.53 kg/hm2 and molybdenum fertilizer 0.65 kg/hm2, the content of 10 nucleosides was optimal. |
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