| 12个地方鸡种遗传多态性的AFLP指纹分析 |
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| 引用本文： | 高玉时,屠云洁,钱勇,李慧芳,陈宽维,童海兵.12个地方鸡种遗传多态性的AFLP指纹分析[J].农业生物技术学报,2006,14(4):498-502. |
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| 作者姓名： | 高玉时 屠云洁 钱勇 李慧芳 陈宽维 童海兵 |
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| 作者单位： | 中国农业科学院家禽研究所,扬州,226003 |
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| 基金项目： | 江苏省自然科学基金;江苏省六大人才高峰基金;江苏省公益性研究项目 |
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| 摘 要： | 利用6对AFLP引物组合对中国12个地方鸡种进行了遗传检测，构建了各个品种的AFLP DNA指纹图谱，根据AFLP分析结果, 统计了每个引物组合在各个品种中检测到的多态性条带和特异性条带，计算了12个地方鸡种的遗传相似系数和遗传距离，并据此构建了UPGMA聚类关系图，分析了所研究鸡种的遗传关系。结果表明：6对AFLP引物组合在12个地方鸡种中共检测到279条多态性条带，平均每个引物组合产生46.5条多态性标记，同时在每个品种群体中还检测到了数量不等的特异性条带，其中寿光鸡和东乡黑鸡最多，为9条，旧院黑鸡和兴义矮脚鸡最少，为1条。12 个鸡种聚为3类，鸡种间的遗传相似系数及聚类结果与所保存的地方鸡种的地理分布、现实状况是相吻合的。从而表明AFLP指纹用于我国地方鸡种遗传多态性分析、品种的鉴定及品种间的亲缘关系分析是可行的。
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| 关 键 词： | 地方鸡种 AFLP指纹分析 品种鉴定 亲缘关系 |
| 文章编号： | 1006-1304（2006）04-0498-05 |
| 收稿时间： | 2005-06-13 |
| 修稿时间： | 2005-08-14 |
AFLP Fingerprinting Analysis of Genetic Polymorphism in 12 Indigenous Chicken Breeds |
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| GAO Yu-shi,TU Yun-jie,QING Yong,LI Hui-fang,CHEN Kuan-wei,TONG Hai-bing.AFLP Fingerprinting Analysis of Genetic Polymorphism in 12 Indigenous Chicken Breeds[J].Journal of Agricultural Biotechnology,2006,14(4):498-502. |
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| Authors: | GAO Yu-shi TU Yun-jie QING Yong LI Hui-fang CHEN Kuan-wei TONG Hai-bing |
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| Institution: | Institute of Poultry, Chinese Academy of Agriculture Sciences, Yangzhou 225003, China |
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| Abstract: | A total of 6 AFLP primer combinations were used to detect genetic variation of pooled DNA in a sample of 12 indigenous chicken breeds, and AFLP DNA fingerprinting of each chicken breed was constructed. Polymorphic bands, specific bands and genetic similarity coefficient of 12 chicken breeds were detected and calculated from AFLP data, and UPGMA cluster analysis was also performed. The 6 primer combinations generated 279 polymorphic bands, 46.5 polymorphic markers were detected by one primer combination on the average. Nine specific bands were produced in the pooled DNA of Shouguang chickens and Dongxiang dark chickens, however, 1 specific band was produced in the pooled DNA of Jiuyuan dark chickens and Xingyi bantam chickens. UPGMA cluster analysis revealed that 12 chicken breeds were divided into three groups. Genetic similarity coefficient and the UPGMA tree of 12 chicken breeds were consistent with its breeding history and geographical distribution. It is feasible to analyze the genetic diversity, genetic relationship and identify chicken breeds based on AFLP DNA fingerprinting. |
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| Keywords: | indigenous chicken breeds AFLP analysis identify breeds genetic relationship |
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