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| 马铃薯遗传资源多样性的SRAP分析 | |
| 引用本文： | 何凤发,杨志平,张正圣,王贵学,王季春.马铃薯遗传资源多样性的SRAP分析[J].农业生物技术学报,2007,15(6):1001-1005. |
| 作者姓名： | 何凤发 杨志平 张正圣 王贵学 王季春 |
| 作者单位： | 1. 西南大学农学与生物科技学院,重庆,400716;重庆大学生物工程学院,重庆,400044 2. 西南大学农学与生物科技学院,重庆,400716 3. 重庆大学生物工程学院,重庆,400044 |
| 摘 要： | 采用SRAP分子标记，对44份马铃薯品种的遗传多样性进行了分析。结果显示, 随机抽取27对SRAP引物，对基因组DNA进行特异扩增，其中23对引物扩增出多态性条带，共获得104个多态性条带, 多态性引物比率达85.2%, 平均每对引物产生4.5个多态性条带，表明SRAP标记具有较高的多态性比率。44份种质资源的SRAP标记遗传距离为0.147-0.741。当遗传距离D=0.67时，将44份种质资源分为四大类群，包括一个复合类群和三个独立类群。其中复合类群可进一步分为7个亚群。从而在DNA水平上证明了所研究马铃薯种质资源具有丰富的遗传多样性。 |
| 关 键 词： | 马铃薯 品种资源 遗传多样性 |
| 文章编号： | 1006-1304（2007）06-1001-05 |
| 收稿时间： | 2007-04-05 |
| 修稿时间： | 2007-05-30 |
Genetic Diversity Analysis of Potato Germplasm by SRAP Makers |
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| HE Feng-fa,YANG Zhi-ping,ZHANG Zheng-sheng,WANG Gui-xue,WANG Ji-chun.Genetic Diversity Analysis of Potato Germplasm by SRAP Makers[J].Journal of Agricultural Biotechnology,2007,15(6):1001-1005. | |
| Authors: | HE Feng-fa YANG Zhi-ping ZHANG Zheng-sheng WANG Gui-xue WANG Ji-chun |
| Abstract: | Sequence related amplified polymorphism(SRAP) markers were employed to analyze the genetic diversity of potato(Solanum tuberosum) varieties. Twenty three out of the randomly selected 27 primer pairs were polymorphic among the 44 varieties tested, and the polymorphism ratio of primers was 85.2%. A hundred and four polymorphism bands were obtained, and the average polymorphism bands were 4.5 per primer pairs. These results demonstrated that SRAP had higher polymorphism in the tested potato varieties. Based on cluster analysis with the SRAP makers, the genetic distance range among the tested 44 varieties was from 0.147 to 0.741. At the genetic distance of 0.67, 44 varieties were clustered into 4 main groups, including one compound group and three discrete ones. Furthermore, the compound group could be subdivided into 7 subgroups. The genetic diversity of the tested potato varieties was thus confirmed at the DNA level. |
| Keywords: | SRAP |
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