| 应用PCR技术检测饲料中的鱼源性成分 |
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| 引用本文： | 贺云霞,王加启,王丽,卜登攀,魏宏阳,胡菡,周凌云.应用PCR技术检测饲料中的鱼源性成分[J].农业生物技术学报,2007,15(6):953-957. |
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| 作者姓名： | 贺云霞 王加启 王丽 卜登攀 魏宏阳 胡菡 周凌云 |
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| 作者单位： | 1. 中国农业科学院北京畜牧兽医研究所,动物营养学国家重点实验室,农业部奶及奶制品质量监督检验测试中心,北京,100094
2. 扬州大学动物科学与技术学院,扬州,225009 |
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| 基金项目： | 农业部无公害农产品质量检测项目 |
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| 摘 要： | PCR方法在动物源性饲料检测中有很好的应用前景。为了有效检测反刍动物饲料中的鱼源性成分，降低疯牛病传播风险，本研究根据鱼线粒体1DNA 6S rRNA序列设计并筛选了鱼源性特异引物，使用Qiagen公司的组织DNA提取试剂盒提取核酸。PCR特异性检测结果表明，引物NC_Fish2480/2501F/ NC_Fish2565/2586R与牛、羊、猪、鸡成分无交叉反应；灵敏性检测结果表明，该引物可以检测到0.1%的鱼源性成分。该方法的建立为饲料中鱼源性成分的PCR检测提供了依据。
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| 关 键 词： | PCR 鱼源性成分 检测 |
| 文章编号： | 1006-1304（2007）06-0953-05 |
| 收稿时间： | 2007-03-28 |
| 修稿时间： | 2007-07-18 |
PCR Method for Detection of Fish-derived Component in Animal Feedstuffs |
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| HE Yun-xia,WANG Jia-qi,WANG Li,BU Deng-pan,WEI Hong-yang,HU Han,ZHOU Ling-yun.PCR Method for Detection of Fish-derived Component in Animal Feedstuffs[J].Journal of Agricultural Biotechnology,2007,15(6):953-957. |
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| Authors: | HE Yun-xia WANG Jia-qi WANG Li BU Deng-pan WEI Hong-yang HU Han ZHOU Ling-yun |
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| Abstract: | Polymerase chain reaction (PCR) is perspective for detection of animal derived feedstuffs. In order to reduce safety risks deriving from bovine spongiform encephalopathy and detect fish-derived material efficiently by PCR method, specific primers were designed according to fish mitochondria 16S rDNA sequence. DNeasy Tissue Kit of Qiagen was applied to extract nucleic acid. Specificity detection results showed that primers NC-Fish2480/2501F and NC-Fish2565/2586R had no cross-reaction with bovine, ovine, porcine and chicken derived material, respectively. Sensitivity results showed that the detection limitation was 0.05% fish derived material. The studies provide a method for PCR detection fish-derived material in ruminant feedstuffs. |
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| Keywords: | PCR |
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