| RAPD分析野生和养殖太湖秀丽白虾的遗传多样性 |
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| 引用本文： | 张敏莹,刘焕章,徐东坡,刘凯,段金荣,施炜纲.RAPD分析野生和养殖太湖秀丽白虾的遗传多样性[J].农业生物技术学报,2008,16(5). |
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| 作者姓名： | 张敏莹 刘焕章 徐东坡 刘凯 段金荣 施炜纲 |
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| 作者单位： | 1. 中国水产科学研究院淡水渔业研究中心,内陆渔业生态环境和资源重点开放实验室,无锡,214081
2. 中国科学院水生生物研究所,武汉,430072 |
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| 基金项目： | 科技部科技基础条件平台建设计划，水产科学研究院专项资金项目 |
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| 摘 要： | 摘要：用60个随机引物对野生和养殖的太湖秀丽白虾进行随机扩增多态DNA(RAPD)分析，筛选的23个随机引物共检测到105个位点（200～2000bp）。用PopGen1.32分析结果表明：野生种群多态位点比例为50.47%，养殖种群多态位点比例为43.33%；野生种群和养殖种群的种群内遗传相似系数分别为0.8635和0.8795；野生种群和养殖种群之间的遗传距离为0.0361；野生种群平均Shannon多样性指数h0为0.2830，略高于养殖种群的0.272。
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| 关 键 词： | 太湖秀丽白虾 野生种群 养殖种群 RAPD 遗传多样性 |
| 收稿时间： | 2007-6-8 |
| 修稿时间： | 2007-8-3 |
RAPD Analysis of Genetic Diversity of Wild and Cultured Exopalaemon modestus in Taihu Lake |
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| ZHANG Min-ying,LIU Huan-zhang,XU Dong-po,LIU Kai,DUAN Jin-rong,SHI Wei-gang.RAPD Analysis of Genetic Diversity of Wild and Cultured Exopalaemon modestus in Taihu Lake[J].Journal of Agricultural Biotechnology,2008,16(5). |
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| Authors: | ZHANG Min-ying LIU Huan-zhang XU Dong-po LIU Kai DUAN Jin-rong SHI Wei-gang |
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| Abstract: | Abstract: The genetic diversity of wild and cultured Exopalaemon modestus in Taihu Lake was analyzed by random amplified polymorphic DNA (RAPD) technique . 23 random primers were selected from 60 random primers ,which could amplify 105 fragments in total with the fragment lenth varing from 200 to 2000 bp. The resuts using PopGen1.32 showed the proportion of polymorphic loci of wild and cultured Exopalaemon modestus was 50.47% and 43.33%,respectively. The intra-population genetic similarity of wild and cultured population was 0.8635 and 0.8795 respectively ,while inter-population genetic distance between the two populations was 0.0361. The mean Shannon index H0 was 0.2830 in wild population ,which was slightly higher than the value 0.272 in cultured population. |
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| Keywords: | RAPD |
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