| 一个棉铃虫触角特异表达基因cDNA片段的克隆 |
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| 引用本文： | 王桂荣,郭予元,吴孔明.一个棉铃虫触角特异表达基因cDNA片段的克隆[J].农业生物技术学报,2003,11(1):49-54. |
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| 作者姓名： | 王桂荣 郭予元 吴孔明 |
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| 作者单位： | 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京,100094 |
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| 基金项目： | 国家重点基础研究发展规划项目(G2000016208). |
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| 摘 要： | 摘要：昆虫触角嗅觉感器的主要功能是识别空气中的挥发性气味分子，它们直接与外界环境接触，外界环境中许多分子对昆虫细胞具有毒性，昆虫触角中必定存在某种机制分解这些毒性物质以及多余的气味刺激物。利用差异显示PCR的方法分离得到了3条棉铃虫(Helicoverpa armigera )触角专一性的mRNA片段，在GenBank中进行同源搜索表明，克隆A4-1与昆虫谷胱甘肽-S-转移酶基因家族具有很高的同源性。Northern杂交表明，克隆A4-1在棉铃虫触角中专一性表达，并且在雄蛾触角中的表达量明显比雌虫中高，这与棉铃虫性外激素结合蛋白基因的表达相似。根据该基因在昆虫触角中的表达情况以及已经报道的谷胱甘肽-S-转移酶在昆虫中所起的作用，推测该基因与性外激素的分解有关，同时可能参与触角中毒性分子的分解。
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| 关 键 词： | 关键词：棉铃虫 差异显示PCR 谷胱甘肽-S转移酶 嗅觉 性外激素 |
| 修稿时间： | 2002年2月28日 |
Cloning of cDNA Fragment of an Antennal-specific Gene in Helicoverpa armigera |
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| Abstract: | Abstract: The olfactory sensors of insects' antenna, which directly exposed to the environment, play an important role in identifying volatile hydrophobic molecules in the air. However, many odors in the air are cytotoxic xenobiotics, so a certain mechanism must exist in insect's antenna to decompose cytotoxic xenobiotics and redundant odorant stimulators. In the present study, differential display polymerase chain reaction (dd-PCR) was used to isolate three antennal -specific mRNA from the moth of cotton bollworm(Helicoverpa armigera ). Sequence analysis indicated that A4-1 clone was homologous to the glutathione S-transferase family of proteins. Northern blot showed that A4-1 clone was specifically expressed in the antenna of H. armigera and the expressed level was higher in male antenna than that in female ones, which was similar to the expression of PBP-Harm gene from cotton bollworm. According to the gene's expression in insects' antenna and the role of glutathione S-transferase reported in other insects, the gene was predicted to be responsible for the decomposition of pheromone and harmful xenobiotics in antenna. |
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