Extraction,purification and properties of soil hydrolases |
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| Authors: | L Batistic JM Sarkar J Mayaudon |
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| Institution: | Laboratoire d''Ecologie Microbienne, Faculté des Sciences Agronomiques Université de Louvain, Place Croix du Sud, 2, 1348-Louvain-la-Neuve, Belgium |
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| Abstract: | Invertase, cellulase, phosphatases, protease and β-glucosidase were extracted from permanent pasture soil with 0.2 M phosphate buffer (pH 8) in the presence of 0.2 M EDTA. This extract was further treated with ammonium and salmine sulphates. Attempts were made to fractionate these enzyme activities by gel and anion-exchange chromatography. Specific activities were estimated in all fractions and some characteristics of the purified enzymes (optimum pH, temperature and substrate concentration, and Km and Vmax) were investigated. The results indicated that extracted enzyme activities occurred partly in soil as a carbohydrate-enzyme complex and partly as a humo-carbohydrate complex. |
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