Cross-amplification and characterization of microsatellite loci for three species of <Emphasis Type="Italic">Theobroma</Emphasis> (Sterculiaceae) from the Brazilian Amazon |
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| Authors: | Maristerra R Lemes Thieme M Martiniano Vanessa M Reis Camila P Faria Rogério Gribel |
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| Institution: | 1.Laboratório de Genética e Biologia Reprodutiva de Plantas,Instituto Nacional de Pesquisas da Amaz?nia,Manaus,Brazil |
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| Abstract: | This study reports on the cross-species amplification of 23 microsatellite markers previously developed for Theobroma
cacao L. (Sterculiaceae), source of chocolate in three economically important Amazonian species of Theobroma (T. grandiflorum, T. subincanum, T. sylvestre). Thirteen of the 23 microsatellite loci tested were polymorphic across the three species at 2–13 alleles per locus. The
observed heterozygosity per locus varied from 0.18 to 0.84 and expected heterozygosity ranged from 0.28 to 0.87. The high
level of transferability and genetic information content of these microsatellite loci indicate their usefulness for population
genetic, mating system and breeding studies of these economically important Amazonian fruit trees. |
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| Keywords: | Amazon Cocoa Genetic diversity SSR Transferability Tropical tree |
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