Development of EST-SSR in foxtail millet (<Emphasis Type="Italic">Setaria italica</Emphasis>) |
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| Authors: | Xiao-Ping Jia Yun-Su Shi Yan-Cun Song Guo-Ying Wang Tian-Yu Wang Yu Li |
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| Institution: | (1) State Key Laboratory of Agri-biotechnology, China Agricultural University, Beijing, 100094, China;(2) Institute of Crop Science, Chinese Academy of Agricultural Sciences, The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Beijing, 100081, China |
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| Abstract: | The development of EST-SSR in foxtail millet (Setaria italica) for polymorphism and transferability study was reported here. From 1213 EST sequences, 30 SSRs were obtained and primers
were designed for 26 SSRs. Among them, four pairs of SSR primers amplified polymorphic products in 12 foxtail millet cultivars
and one accession of Setaria viridis, a wild relative of foxtail millet, with 10 alleles detected for the four loci and 2.5 alleles per locus. In addition, ten
SSR markers could be transferred to other nine Gramineae species. The putative functions of 11 ESTs containing polymorphic
and transferable SSRs were also identified. |
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| Keywords: | EST-SSR Foxtail millet Polymorphism Setaria italica Transferability |
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