Low genetic diversity in wild emmer (T. turgidum L. subsp. dicoccoides (Körn. ex Asch. et Graebn.) Thell.) from South-eastern Turkey revealed by Restriction Fragment Length Polymorphism |
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| Authors: | Bahattin Tanyolac Eric Linton Hakan Ozkan |
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| Institution: | (1) Department of Bioengineering, Ege University, 35100 Bornova, Izmir, Turkey;(2) Waksman Institute of Microbiology, The State University of New Jersey, Rutgers, US;(3) Department of Field Crops, Cukurova University, Balcali, Turkey |
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| Abstract: | The present work was carried out to study genetic diversity among 17 populations of wild emmer wheat sampled from South-eastern Turkey, considered to be an important region for domestication of wheat. Eleven RFLP clones and 4 restriction enzymes combinations were used to probe the genomic DNA. A total of 151 polymorphic loci were obtained from the enzyme-probe combinations. The Genetic Distance (GD) values were from 0.019 (Gaziantep-3 and Sanliurfa-4) to 0.200 (Gaziantep-1 and K. Maras). Cluster analysis results showed that populations formed 2 clades within the dendrogram. Population Gaziantep-1 was unique and genetically most diverse from the remaining 16 populations. The results of average genetic distance (GD) among populations suggested that narrow genetic variability exist among 17 populations in the present study. |
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| Keywords: | Genetic polymorphisms Genetic resources RFLP Wild emmer wheat |
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