| 外源NO对盐胁迫下甜高粱种子萌发和幼苗生长的影响 |
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| 引用本文： | 王旺田,谢光辉,刘文瑜,王宝强,郑凯翔,魏晋梅.外源NO对盐胁迫下甜高粱种子萌发和幼苗生长的影响[J].核农学报,2019,33(2):363-371. |
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| 作者姓名： | 王旺田 谢光辉 刘文瑜 王宝强 郑凯翔 魏晋梅 |
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| 作者单位： | 1 甘肃农业大学生命科学技术学院/甘肃省干旱生境作物学重点实验室,甘肃 兰州 730070; 2 中国农业大学农学与生物技术学院,北京 100193; 3 甘肃省农业科学院畜草与绿色农业研究所,甘肃 兰州 730070; |
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| 基金项目： | 国家自然基金(31560552) |
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| 摘 要： | 为探究外源NO对盐胁迫下甜高粱种子萌发和幼苗生长的影响,以国能4号甜高粱为试验材料,采用不同浓度(0.05、0.1和0.2 mmol·L-1)硝普钠(SNP,NO供体)处理1.6%NaCl胁迫下的种子和幼苗,统计种子发芽数,测定幼苗叶片叶绿素、脯氨酸、可溶性糖、可溶性蛋白、丙二醛(MDA)含量及超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性等生理生化指标。结果表明,盐胁迫抑制了甜高粱种子的萌发,种子发芽指标(种子发芽率、发芽势和发芽指数)显著降低,1.6%NaCl是甜高粱种子萌发的敏感盐浓度;喷施不同浓度SNP可显著提高盐胁迫下甜高粱种子的发芽率及幼苗叶片叶绿素、可溶性糖、可溶性蛋白和脯氨酸含量,降低叶片MDA含量,增强抗氧化酶活性,其中0.1 mmol·L-1SNP处理效果最佳。0.1 mmol·L-1SNP处理下,与单独盐浓度处理相比,甜高粱种子发芽率、发芽势和发芽指数分别增加了29.51%、39.21%和38.91%;幼苗叶片叶绿素a、叶绿素b、总叶绿素、可溶性糖、可溶性蛋白和脯氨酸含量分别提高了230.00%、184.38%、214.13%、17.00%、8.78%和40.63%;MDA含量降低了34.01%;SOD、POD、CAT和APX活性分别增强了33.38%、55.75%、23.17%和116.46%。综上,盐胁迫下适宜浓度的SNP处理,可提高甜高粱幼苗叶片渗透调节物质含量,增强抗氧化酶活性,清除体内活性氧,从而促进幼苗生长,增强植株抗盐性。本研究结果为提高甜高粱耐盐性及揭示其耐盐机制提供了理论依据。
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| 关 键 词： | 甜高粱 一氧化氮 盐胁迫 种子萌发 抗氧化酶 |
| 收稿时间： | 2017-12-05 |
Effect of Exogenous Nitric Oxide on Seed Germination and Seedling Growth of Sorghum Under Salt Stress |
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| WANG Wangtian,XIE Guanghui,LIU Wenyu,WANG Baoqiang,ZHENG Kaixiang,WEI Jinmei.Effect of Exogenous Nitric Oxide on Seed Germination and Seedling Growth of Sorghum Under Salt Stress[J].Acta Agriculturae Nucleatae Sinica,2019,33(2):363-371. |
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| Authors: | WANG Wangtian XIE Guanghui LIU Wenyu WANG Baoqiang ZHENG Kaixiang WEI Jinmei |
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| Institution: | 1 Gansu Provincial Key Laboratory of Arid Land Group Science/College of Life Science and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070; 2 College of Agronomy, China Agricultural University, Beijing 100193; 3 Institute of Pasture and Green Agriculture, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu 730070; |
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| Abstract: | In order to study effect of exogenous nitric oxide on seed germination and seedling growth of Sorghum under salt stress, we used Sorghum variety Guoneng No.4 as experimental materials, it was pretreated with 0.05, 0.1 and 0.2 mmol·L-1 sodium nitriprusside SNP, a nitric oxide (NO) donor]. The results showed that seed germination ability was inhibited by salt stress, and 1.6% NaCl is the sensitive concentration of salt stress to Sorghum seeds. Damage to Sorghum seedlings caused by 1.6% NaCl stress was significantly alleviated in the presence of different concentrations of SNP, and 0.1 mmol·L-1 SNP was the best choice. Compared with single NaCl treatment, seed germination rate, germination power and germination indexes were increased by 29.51%, 39.21% and 38.91%,respectively. The contents of chl a, chl b, chlorophyll, soluble sugar, soluble protein and proline were increased by 230.00%, 184.38%, 214.13%, 17.00%, 8.78% and 40.63%, respectively. The contents of MDA were decreased by 34.01%. The activities of SOD, POD, CAT and APX were increased by 33.38%, 55.75%, 23.17% and 116.46%, respectively. In conclusion, suitable concentration of SNP may alleviate damage to seeds and seedlings caused by salt stress. Key components of the response are: increased the osmotic adjustments level, enhanced antioxidant enzyme activities, decreased the reactive oxygen species level, with resultant promotion of the seedling growth, and enhanced the salt resistant ability of Sorghum. |
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| Keywords: | sorghum nitric oxide salt stress seed germination antioxidative enzymes |
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