| 山西芝麻种质资源SSR遗传多样性及群体结构分析 |
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| 引用本文： | 吕伟,韩俊梅,任果香,文飞,王若鹏,刘文萍.山西芝麻种质资源SSR遗传多样性及群体结构分析[J].核农学报,2021,35(7):1495-1506. |
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| 作者姓名： | 吕伟 韩俊梅 任果香 文飞 王若鹏 刘文萍 |
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| 作者单位： | 山西农业大学经济作物研究所,山西太原030031 |
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| 基金项目： | 国家特色油料产业技术体系(CARS-14-2-04),山西省科技攻关项目(201703D221007-1),山西农业大学育种工程项目(17 yzgc 050),山西农业大学院有机旱作农业研发专项(YCX2018419) |
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| 摘 要： | 为探明山西芝麻种质资源的遗传特性,本研究利用30对简单重复序列标记(SSR)对71份山西芝麻种质资源进行遗传多样性分析及群体结构分析。结果表明,30对SSR标记共检测到144个等位基因位点,平均每个SSR标记4.800个等位基因;有效等位基因数在1.058~5.149之间,平均2.805个;Shannon指数变幅为0.128~1.813,平均为1.096;Nei's遗传多样性指数变幅为0.055~0.806,平均为0.558;多态性信息含量变幅为0.053~0.783,平均为0.515。基于SSR标记对参试材料进行聚类分析,遗传相似系数为0.21~0.67,在遗传相似系数0.27处将参试材料分为6个类群;基于SSR标记对参试材料进行群体结构分析,将参试材料划分为5个组群。综上所述,山西芝麻种质资源间遗传差异相对较高,具有丰富的遗传多样性,在今后芝麻种质资源创制利用中,加大山西芝麻种质资源的开发与利用,可为芝麻品种遗传改良和优异基因发掘奠定良好的基础。
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| 关 键 词： | 芝麻 SSR标记 遗传多样性 群体结构分析 |
| 收稿时间： | 2019-08-13 |
Genetic Diversity Analysis of Sesame Germplasm Resourcesin Shanxi With SSR Markers |
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| LYU Wei,HAN Junmei,REN Guoxiang,WEN Fei,WANG Ruopeng,LIU Wenping.Genetic Diversity Analysis of Sesame Germplasm Resourcesin Shanxi With SSR Markers[J].Acta Agriculturae Nucleatae Sinica,2021,35(7):1495-1506. |
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| Authors: | LYU Wei HAN Junmei REN Guoxiang WEN Fei WANG Ruopeng LIU Wenping |
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| Institution: | Industrial Crops Institute of Shanxi Agricultural University, Taiyuan, Shanxi 030031 |
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| Abstract: | In order to explore the genetic characteristics of sesame germplasm resources in Shanxi, 30 pairs of simple sequence repeat (SSR) were used to analyze the genetic diversity and population structure of 71 sesame germplasms of Shanxi in this study. The results showed that a total of 144 allelic loci were detected with 30 pairs of SSR markers, the mean number of alleles per locus was 4.800; the number of effective alleles ranged from 1.058 to 5.149, with an average of 2.805; the index of Shannon ranged from 0.128 to 1.813, with an average of 1.096; the genetic diversity index of Nei's varied from 0.055 to 0.806, with an average of 0.558; the polymorphic information content ranged from 0.053 to 0.783, with an average of 0.515. Based on cluster analysis with SSR molecular markers, the genetic similarity coefficient of the tested materials was 0.21~0.67, and six groups were clustered at the genetic similarity coefficient of 0.27; The population structure analysis based on SSR molecular markers divided the test materials into five groups. The results showed that the genetic difference among sesame germplasm resources in Shanxi was relatively high and there were rich genetic diversities. Development and utilization of sesame germplasm resources of Shanxi might lay a good foundation for the genetic improvement and discovery of excellent genes of sesame in future. |
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| Keywords: | sesame SSR markers genetic diversity group structure analysis |
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