| 杂交技术培育金针菇尿嘧啶营养缺陷型双核体菌株 |
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| 引用本文： | 鲍大鹏,万佳宁,卢绪志,余养朝,张光忠,茅文俊,王莹,周陈力.杂交技术培育金针菇尿嘧啶营养缺陷型双核体菌株[J].核农学报,2020,34(2):225-230. |
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| 作者姓名： | 鲍大鹏 万佳宁 卢绪志 余养朝 张光忠 茅文俊 王莹 周陈力 |
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| 作者单位： | 1上海市农业科学院食用菌研究所/农业农村部南方食用菌资源利用重点实验室/国家食用菌工程技术研究中心/上海市农业遗传育种重点开放实验室,上海 201403; 2 江苏华绿生物科技股份有限公司,江苏 泗阳 223700 |
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| 基金项目： | 上海市科委工程技术研究中心能力提升项目 |
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| 摘 要： | 为培育金针菇尿嘧啶营养缺陷型双核体菌株,将金针菇尿嘧啶营养缺陷型单核体菌株NG1-65、NG1-92和NG1-95(A1B1)分别与可亲和的野生型单核菌株DG1-29(A2B2)杂交得到3株杂交菌株SGN1、SGN2和SGN3,通过栽培试验获得这3株双核体菌株的子实体,收集孢子后通过涂布于选择培养基和镜检获得206株尿嘧啶营养缺陷型单核体菌株,从中随机选取30株单核体菌株,通过单单杂交得到38株尿嘧啶营养缺陷型双核体菌株。结果表明,这些尿嘧啶营养缺陷型双核体菌株在不含尿嘧啶添加物的基本培养基中无法正常生长,在PDA培养基上生长速率低于野生型菌株,在添加尿嘧啶的PDAU培养基上可以不同程度地恢复正常生长。本研究结果为进一步利用营养缺陷型菌株开展金针菇杂交育种、菌种保护等方面研究提供了一定的技术支撑。
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| 关 键 词： | 金针菇 尿嘧啶营养缺陷型 双核体 菌种保护 |
| 收稿时间： | 2018-07-27 |
Establishment of Uracil Auxotrophic Dikaryotic Strains of Flammulina velutipes by Crossbreeding |
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| BAO Dapeng,WAN Jianing,LU Xuzhi,YU Yangchao,ZHANG Guangzhong,MAO Wenjun,WANG Ying,ZHOU Chenli.Establishment of Uracil Auxotrophic Dikaryotic Strains of Flammulina velutipes by Crossbreeding[J].Acta Agriculturae Nucleatae Sinica,2020,34(2):225-230. |
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| Authors: | BAO Dapeng WAN Jianing LU Xuzhi YU Yangchao ZHANG Guangzhong MAO Wenjun WANG Ying ZHOU Chenli |
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| Institution: | 1Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences/Key Laboratory of Applied Mycological Resources and Utilization,(south), Ministry of Agriculture and Rural Affairs/Laboratory of Edible Fungi Genetic, National Engineering Research Center of Edible Fungi/Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Shanghai 201403; 2Jiangsu Chinagreen Biological Technology Co.,Ltd, Siyang, Jiangsu 223700 |
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| Abstract: | To cultivate the uracil auxotrophic dikaryon strains, three uracil auxotrophic monokaryon strains of F. velutipes NG1-65,NG1-92 and NG1-95 (A1B1) were crossed with the wild type monokaryon DG1-29(A2B2) respectively, and three dikaryotic hybrids SGN1, SGN2 and SGN3 were produced. 206 auxotrophic monokaryon strains were obtained by spreading spores on selective medium and microscopic examination. Among them, 30 strains were randomly selected and used as parents to produce uracil auxotrophic dikaryon strains. 38 strains were obtained by Monkaryon-monkaryon crossing. Tests showed these strains did not grow on minimal medium and had slower growth rates on PDA plate comparing to wild type. The PDAU mediums with uracil restored their growth to varying degrees. This results of the study would will be helpful to mushroom breeding and spawn protection. |
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| Keywords: | Flammulina velutipes uracil auxotroph dikaryon spawn protection |
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