| 固氮粪产碱菌（Alcaligenes faecalis）胞外多糖的分离纯化及成分分析 |
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| 引用本文： | 方宣钧,林敏,尤崇杓.固氮粪产碱菌（Alcaligenes faecalis）胞外多糖的分离纯化及成分分析[J].核农学报,1995,9(4):0-0. |
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| 作者姓名： | 方宣钧 林敏 尤崇杓 |
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| 作者单位： | 中国农业科学院原子能利用研究所 |
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| 摘 要： | 固氨粪产碱菌野生型Ａ１５０１菌株及其胞外多糖（ＥＰＳ）突变株Ｅｘｏ＋＋（Ａ１５３２）和ＥｘＯ－（Ａ１５３１）,以及ｎｉｆＡ和ｎｔｒＣ－ｎｉｆＡ转化子（Ａ１５１３和Ａ１５２３）的ＥＰＳ,由高分子量（２５０ｋＤ左右）组分（ＥＰＳＨ）和低分子量小于４０ｋＤ组分（ＥＰＳＬ）组成。两个组分中均含有葡萄糖、半乳糖、果糖和戊糖,其主要成分葡萄糖和半乳糖的分子摩尔比：Ａ１５０１－ＥＰＳＨ为２：１、ＥＰＳＬ为３：１;Ａ１５３１－ＥＰＳＨ为２：１、ＥＰＳＬ为１：１;Ａ１５３２－ＥＰＳＨ为８：１、ＥＰＳＬ为５：１;Ａ１５１３－ＥＰＳＨ和ＥＰＳＬ均为４：１;Ａ１５２３－ＥＰＳＨ为４：１、ＥＰＳＬ为８：１。高分子量ＥＰＳ中还含有多肤,其氨基酸组分在各突变株和转化子之间差异明显,而ＥＰＳＬ中仅含有痕量多肽。
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| 关 键 词： | 粪产碱菌 胞外多糖 分离 成分分析 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2009-12-31 |
PURIFICATION AND ANALYSIS OF EXOPOLYSACCHARIDES FROM Alcaligenes faecalis AND ITS MUTANTS |
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| Abstract: | The exotracelluar polysacchrides (EPS )were isolated and purifled from A. faecalisA1501 and its mutants A1513,A1532,A1513,and A1523.EPS of these bacteria contained sugar and polypeptide and consists of two distinct fractions:EPS component of high molecu-lar weight and low molecular weight.The molecular weight of HMW component was 250kD,and that of LMW component was less than 40kD. Both of them contained glucose, galactose,fructose and some pentose,and the ratio of glucose,galactose for wild type and its mutantswas as follows :A1501 :EPSH 2 :1 , EPSL3:1;A1513EPSH2 : 1 , EPSL1:1; A1532 EPSH8:1 ,EPSL5:1;A1513 EPSH,EPSL 4:1; and A5123EPH 4: 1,EPSL8:1.There was apolypeptide component in the HMW fraction. The amino acid compositions were differentamong tbe tested strains. |
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| Keywords: | " target="_blank">Alcaligenes faecalis zz')" href="#"> Alcaligenes faecalis exopolysaccharides(EPS) characters |
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