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| 外源lea3基因转化紫花苜蓿的研究 | |
| 引用本文： | 王瑛,朱宝成,孙毅,张琳宇,罗建平.外源lea3基因转化紫花苜蓿的研究[J].核农学报,2007,21(3):249-252,260. |
| 作者姓名： | 王瑛 朱宝成 孙毅 张琳宇 罗建平 |
| 作者单位： | 1. 合肥工业大学生物与食品工程学院,安徽,合肥,230009;河北大学生命科学学院,河北,保定,071002 2. 河北大学生命科学学院,河北,保定,071002;河北农业大学生命科学学院,河北,保定,071001 3. 河北大学生命科学学院,河北,保定,071002 4. 合肥工业大学生物与食品工程学院,安徽,合肥,230009 |
| 基金项目： | 河北大学校科研和教改项目 |
| 摘 要： | 将来源于大麦的lea3基因通过基因枪法导入紫花苜蓿栽培品种“中苜一号”的胚性愈伤组织中,经过膦丝菌素类除草剂(PPT)的4次筛选获得抗性愈伤组织,诱导分化后共获得21株抗性再生植株。经叶片涂抹除草剂试验和lea3基因的PCR分子检测证明,lea3基因已整合到紫花苜蓿的基因组中。与对照植株相比,获得的转基因植株具有显著增强的耐盐能力,表明遗传转化lea3基因可用于苜蓿抗旱耐盐新品种的选育。 |
| 关 键 词： | 紫花苜蓿 胚性愈伤组织 lea3基因 转化 |
| 文章编号： | 1000-8551（2007）03-249-04 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2006-07-25 |
TRANSFORMATION OF BARELY lea3 GENE INTO ALFALFA (Medicago sativa L.) |
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| WANG Ying,ZHU Bao-cheng,SUN Yi,ZHANG Lin-yu,LUO Jian-Ping.TRANSFORMATION OF BARELY lea3 GENE INTO ALFALFA (Medicago sativa L.)[J].Acta Agriculturae Nucleatae Sinica,2007,21(3):249-252,260. | |
| Authors: | WANG Ying ZHU Bao-cheng SUN Yi ZHANG Lin-yu LUO Jian-Ping |
| Institution: | 1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui 230009 ; 2. College of Life Sciences, Hebei University, Baoding , Hebei , 071002; 3. Collenge of Life Sciences, Hebei Agriculture University, Banding, Hebei 071001 |
| Abstract: | This paper reported the transformation of a late embryogenesis abundant protein gene,lea3,from barely(Hordeum vulgare L.) into Medicago sativa L.Embryogenic callus from Medicago sativa L.cultivar "ZhongMu No.1" was transformed by particle bombardment with plasmid pBY520 containing lea3 gene.After 4 repeated PPT selecting cultures,resistant calli and 21 regenerated plants were obtained.PPT resistance tests and PCR amplification analysis showed that 2 putative transgenic plants carried the lea3 gene.Compared with control plants,transgenic plants had an enhanced salt-tolerance,suggesting that transformation of lea3 gene could be used to select elite alfalfa cultivars with enhanced tolerance to drought and salt stress. |
| Keywords: | Alfalfa(Medicago sativa L ) embryogenic calli lea3 gene transformation |
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