| 不同生长素类型及ABA搭配对小麦幼胚再生效果的影响 |
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| 引用本文： | 别晓敏,杜丽璞,佘茂云,徐惠君,叶兴国.不同生长素类型及ABA搭配对小麦幼胚再生效果的影响[J].核农学报,2011,25(5):1023-1028. |
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| 作者姓名： | 别晓敏 杜丽璞 佘茂云 徐惠君 叶兴国 |
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| 作者单位： | 中国农业科学院作物科学研究所／农作物基因资源与基因改良国家重大科学工程／农业部作物遗传育种重点开放实验室,北京,100081 |
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| 基金项目： | 国家自然科学基金(30971776); 国家转基因研究专项(2008ZX08010-004) |
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| 摘 要： | 生长素是小麦幼胚产生胚性愈伤组织的关键成分,培养基中适宜的生长素种类、浓度和组合决定着小麦幼胚再生效果。本研究以小麦基因型CB037幼胚为材料,在分别确定dicamba、2,4,5-T和picloram的适宜用量的基础上,比较了2,4-D、dicamba、2,4,5-T、picloram在适宜浓度下诱导再生的效果,筛选...
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| 关 键 词： | 小麦 幼胚 生长素 胚性愈伤组织诱导率 植株再生率 |
| 收稿时间： | 2010-01-11 |
| 修稿时间： | 2011-04-21 |
EFFECTS OF DIFFERENT AUXINS AND COMBINING APPLICATION WITH ABA ON REGENERATION OF IMMATURE EMBRYOS OF WHEAT |
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| BIE Xiao-min DU Li-pu SHE Mao-yun XU Hui-jun YE Xing-guo.EFFECTS OF DIFFERENT AUXINS AND COMBINING APPLICATION WITH ABA ON REGENERATION OF IMMATURE EMBRYOS OF WHEAT[J].Acta Agriculturae Nucleatae Sinica,2011,25(5):1023-1028. |
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| Authors: | BIE Xiao-min DU Li-pu SHE Mao-yun XU Hui-jun YE Xing-guo |
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| Institution: | BIE Xiao-min DU Li-pu SHE Mao-yun XU Hui-jun YE Xing-guo (Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/National Key Facility of Crop Gene Resources and Genetic Improvement/Key Laboratory of Crop Genetic and Breeding,Ministry of Agriculture,Beijing 100081) |
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| Abstract: | It is necessary to improve plant regeneration efficiency for cell and genetic engineering breeding of wheat.To investigate appropriate type and concentration of auxins(dicamba,2,4,5-T,picloram) and combination of 2,4-D and ABA for wheat immature embryo culture, a new wheat line CB037 was applied to investigate the influence of three auxins and combination effect of 2,4-D and ABA.Results showed that the optimal concentrations of dicamba,2,4,5-T,picloram when used alone were 2.5mg/L,3.0mg/L and 3.0mg/L,respec... |
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| Keywords: | wheat immature embryos auxins embryogenic callus plant regeneration |
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