| 大白菜抽薹相关基因BrFLC1的KASP标记开发 |
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| 引用本文： | 杨双娟,王志勇,赵艳艳,魏小春,原玉香,张晓伟.大白菜抽薹相关基因BrFLC1的KASP标记开发[J].核农学报,2020,34(2):265-272. |
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| 作者姓名： | 杨双娟 王志勇 赵艳艳 魏小春 原玉香 张晓伟 |
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| 作者单位： | 河南省农业科学院园艺研究所,河南 郑州 450002 |
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| 基金项目： | 农业部黄淮海(河南)蔬菜观测站(10205020);国家重点研发计划;创新项目;河南省科技攻关计划 |
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| 摘 要： | 为了提高大白菜耐抽薹品种的分子育种效率,本研究针对大白菜抽薹相关基因BrFLC1第6个内含子的第一个碱基(Pi6+1)G-A的SNP变异开发进行高通量检测的竞争性等位基因特异性PCR(KASP)标记。结果表明,开发的KASP标记BrFLC1-KASP1可有效将晚抽薹类型材料Y177-12(G)和早抽薹类型材料Y195-93(A)分为2组。利用BrFLC1-KASP1标记可以对57份大白菜材料的基因型进行有效鉴别,且鉴定结果与酶切扩增多态性标记(CAPS)G-MvaI以及直接测序法的鉴定结果完全一致。综上所述, BrFLC1-KASP1标记在大白菜材料中具有通用性,且具有准确率高、成本低、效率高的特点。本研究结果对大白菜耐抽薹性的分子标记辅助选择具有重要的育种实践价值。
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| 关 键 词： | 大白菜 抽薹时间 BrFLC1基因 KASP标记 |
| 收稿时间： | 2018-08-03 |
Development of KASP Marker for Bolting Related Gene BrFLC1 in Chinese Cabbage(Brassica rapa L. ssp. pekinensis) |
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| YANG Shuangjuan,WANG Zhiyong,ZHAO Yanyan,WEI Xiaochun,YUAN Yuxiang,ZHANG Xiaowei.Development of KASP Marker for Bolting Related Gene BrFLC1 in Chinese Cabbage(Brassica rapa L. ssp. pekinensis)[J].Acta Agriculturae Nucleatae Sinica,2020,34(2):265-272. |
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| Authors: | YANG Shuangjuan WANG Zhiyong ZHAO Yanyan WEI Xiaochun YUAN Yuxiang ZHANG Xiaowei |
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| Institution: | Institute of Horticulture,Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002 |
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| Abstract: | In order to improve the efficiency of molecular marker assisted breeding for bolting-resistant varieties in Chinese cabbage (Brassica rapa L. ssp. pekinensis) , we dedicated to develop a KASP (Kompetitive allele specific PCR) marker for high-throughput detection of the G-A variation at the first base in intron 6 (Pi6+1) of gene BrFLC1, which is significantly associated with bolting time phenotype. It turned out that the developed KASP marker BrFLC1-KASP1can successfully differentiate the late-bolting genotype Y177-12 (G) from the early-bolting genotype Y195-93 (A). The genotypes of BrFLC1 gene in 57 accessions were successfully identified by marker BrFLC1-KASP1. In addition, the genotypes of 57 accessions identified by marker BrFLC1-KASP1 were all same with that identified by CAPS (Cleaved Amplified Polymorphic Sequence) marker G-MvaI and direct sequencing, which suggested that this marker is possessed of stronger applicability and generality in Chinese cabbage, furthermore, it is much more economic and efficient. Thus, the marker BrFLC1-KASP1developed in this study can be used for marker-assisted selection in bolting-resistance breeding with lower cost and higher efficiency, which has pivotal significance in breeding practice. |
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| Keywords: | Chinese cabbage bolting time BrFLC1 gene KASP marker |
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