| 2种紫花苜蓿耐盐生理特性的初步研究 |
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| 引用本文： | 黄婷,麻冬梅,王文静,赵丽娟,马巧利,蔡进军.2种紫花苜蓿耐盐生理特性的初步研究[J].水土保持学报,2020,34(2):216-221. |
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| 作者姓名： | 黄婷 麻冬梅 王文静 赵丽娟 马巧利 蔡进军 |
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| 作者单位： | 1. 宁夏大学西北土地退化与生态系统恢复省部共建国家重点实验室培育基地, 西北退化生态系统恢复与重建教育部重点实验室, 银川 750021;2. 宁夏大学农学院, 银川 750021;3. 宁夏农林科学院农业资源环境研究所, 银川 750021;4. 西北农林科技大学资源与环境学院, 陕西 杨凌 712100 |
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| 基金项目： | 国家自然科学基金项目（31760698）；宁夏重点研发计划项目（2019BBF02022-04）；宁夏高等学校一流学科建设（生态学）项目（NXYLXK2017B06） |
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| 摘 要： | 为研究2种紫花苜蓿(Medicagosativa L.)“阿迪娜”(耐盐基因型)和“秘鲁”(敏盐基因型)在盐胁迫处理下的生理特性与耐盐机理,采用150mmol/LNaCl胁迫处理2种紫花苜蓿幼苗,分别测定盐处理前和处理后2,4,6,8,16h的过氧化氢酶(CAT)、过氧化物酶(POD)、丙二醛(MDA)、脯氨酸(PRO)、相对含水量(RWC)和叶绿素(Chl)含量。结果表明:2种紫花苜蓿的过氧化氢酶活性、过氧化物酶活性和丙二醛含量在盐胁迫下总体呈上升趋势,脯氨酸含量和相对含水量表现出相反的趋势。叶绿素含量在盐胁迫前期稳定不变,但在胁迫后期显著性下降;2种紫花苜蓿的相对含水量和叶绿素含量的变化趋势与植株表型变化相对应。主成分分析表明,过氧化氢酶和丙二醛的贡献率最大,能更好地为苜蓿耐盐机理及分子育种研究提供理论依据。
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| 关 键 词： | 紫花苜蓿 盐胁迫 生理指标 耐盐机理 |
| 收稿时间： | 2019/11/23 0:00:00 |
Preliminary Study on Physiological Characteristics of Salt Tolerance of Two Genotypes of Alfalfa |
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| HUANG Ting,MA Dongmei,WANG Wenjing,ZHAO Lijuan,MA Qiaoli,CAI Jinjun.Preliminary Study on Physiological Characteristics of Salt Tolerance of Two Genotypes of Alfalfa[J].Journal of Soil and Water Conservation,2020,34(2):216-221. |
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| Authors: | HUANG Ting MA Dongmei WANG Wenjing ZHAO Lijuan MA Qiaoli CAI Jinjun |
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| Institution: | 1. Breeding Base for State Key Laboratory of Land Degradation and Ecological Restoration in Northwest China, Key Laboratory for Restoration and Reconstruction of Degraded Ecosystem in Northwest China of Ministry of Education, Ningxia University, Yinchuan 750021;2. Agricultural College of Ningxia University, Yinchuan 750021;3. Institute of Agricultural Resources and Environment, Ningxia Academy of Agricultural and Forestry Sciences, Yinchuan 750021;4. College of Resources and Environment, Northwest A & F University, Yangling, Shaanxi 712100 |
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| Abstract: | The physiological characteristics and salt tolerance mechanism of two genotypes of alfalfa (Medicago sativa L.) Adrenalin (salt-stress tolerant) and Peru (salt-stress sensitive) under salt stress were explored by using 150 mmol/L NaCl to deal with two genotypes of alfalfa seedlings. Catalase (CAT), malondialdehyde (MDA), peroxidase (POD), relative water content (RWC), Chlorophyll (Chl), and proline (PRO) were respectively determined before and after 2, 4, 6, 8, 16 hour of salt stress. The result showed that the catalase activity, the peroxidase activity, and the malondialdehyde content of two genotypes of alfalfa showed a trend of increase under salt stress, while the proline content and the relative water content showed an opposite trend. The chlorophyll content significantly decreased. In addition, it was found that the variation of the relative water content and the chlorophyll content corresponded to the change of plant phenotype. Principal component analysis showed that the catalase and the malondialdehyde contributed the most, which could provide a theoretical basis for the research on salt tolerance mechanism and molecular breeding of alfalfa. |
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| Keywords: | alfalfa salt stress physiological indices salt tolerance mechanism |
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