| PCR技术在快速检测沙门氏菌中的应用 |
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| 引用本文： | 沈兰,王锐萍,史海涛,庞贤鹏.PCR技术在快速检测沙门氏菌中的应用[J].广西农业生物科学,2012,31(1):90-94. |
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| 作者姓名： | 沈兰 王锐萍 史海涛 庞贤鹏 |
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| 作者单位： | 海南师范大学生命科学学院,海口,571158 |
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| 基金项目： | 国家自然科学基金重大国际合作项目（30910103916）资助 |
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| 摘 要： | 本文总结了PCR检测沙门氏菌过程中被检目标基因的选择、引物的特异性以及国内外检测沙门氏菌的一些实例,分析了PCR检测沙门氏菌在引物选择上存在的问题,简述了在传统PCR技术基础上发展起来的检测沙门氏菌新方法。
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| 关 键 词： | 沙门氏菌 被检目标基因 引物特异性 PCR技术 |
Applications of PCR Technologies on Rapid Detection of Salmonella |
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| Shen Lan Wang Ruiping Shi Haitao Pang Xianpeng.Applications of PCR Technologies on Rapid Detection of Salmonella[J].Journal of Guangxi Agricultural and Biological Science,2012,31(1):90-94. |
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| Authors: | Shen Lan Wang Ruiping Shi Haitao Pang Xianpeng |
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| Institution: | Shen Lan Wang Ruiping Shi Haitao Pang Xianpeng Centre for Viticulture ＆ Enology,College of Life Sciences,Hainan Normal University,Haikou,571158 |
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| Abstract: | This paper summarized the target gene selection and primer specificity in the PCR procedures of Salmonella detection.Some practical case studies at home and abroad were listed as examples.We also analyzed the problems happened on primer choosing in the PCR detection of Salmonella and introduced some modified approached for Salmonella detection developed based on traditional PCR technologies. |
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| Keywords: | Salmonella Target gene Primer specificity PCR technique |
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