| 水稻转基因整合模式中外源基因的遗传规律 |
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| 引用本文： | 华志华,朱雪峰,吴明国,于彦春,赵艳,高振宇,颜美仙,钱前,黄大年.水稻转基因整合模式中外源基因的遗传规律[J].作物学报,2003,29(1):44-48. |
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| 作者姓名： | 华志华 朱雪峰 吴明国 于彦春 赵艳 高振宇 颜美仙 钱前 黄大年 |
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| 作者单位： | 中国水稻研究所农业部水稻生物学重点实验室,浙江杭州,310006 |
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| 基金项目： | 浙江省自然科学基金 (3 982 2 7)，浙江省生物技术重点攻关项目 (96110 2 2 16)资助项目 |
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| 摘 要： | 通过对低拷贝数的转基因株京引119的世代连续跟踪分析以及高世代材料的群体遗传分析表明, 共转化基因bar和cecropin B在基因组中紧密连锁并能稳定的遗传和表达. 来自于转基因嘉59和丙9363的两高世代抗除草剂材料TR5和TR6的杂交后代分析结果显示, 多拷贝的bar基因以单基因表型性状传递, 两材料中的bar基因互不等位. TR5基因组
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| 关 键 词： | 水稻(Oryza sativa L.) cecropin B bar 遗传 表达部分插入位点 |
| 收稿时间： | 2002-03-05 |
| 修稿时间： | 2002年3月5日 |
Inheritance of Exo-genes Integrated into the Rice Genomes |
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| HUA Zhi,Hua,ZHU Xue,Feng,WU Ming,Guo,YU Yan,Chun,ZHAO Yan,GAO Zhen,YuYAN Mei,Xian,QIAN Qian,HUANG Da,Nian.Inheritance of Exo-genes Integrated into the Rice Genomes[J].Acta Agronomica Sinica,2003,29(1):44-48. |
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| Authors: | HUA Zhi Hua ZHU Xue Feng WU Ming Guo YU Yan Chun ZHAO Yan GAO Zhen YuYAN Mei Xian QIAN Qian HUANG Da Nian |
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| Abstract: | Co transformed genes, bar and cecropin B, which integrated in the genome of Jingyin 119 with low copies, were detected to be linkage and inherit and express stably among different generations. However, according to the cross between TR5 and TR6, two high BASTA resistant generations self pollinated from transgenic Jia59 and Bing9363, the bar genes of multi inserted loci were not alleic. Some bar or cecropin B genes might lose during the multiplication of transgenic plants, but loss did not affect the expression of the transgene. So the phenotype stability could not suggest that the multi inserted loci of transgenes were homozygous. |
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| Keywords: | Rice( Oryza sativa L ) cecropin B bar Heredity Inheritance |
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