| 水稻紫鞘染色体片段代换系Z519的鉴定及PSH1候选基因分析 |
| |
| 引用本文： | 周可,李燕,王世明,崔国庆,杨正林,何光华,凌英华,赵芳明.水稻紫鞘染色体片段代换系Z519的鉴定及PSH1候选基因分析[J].作物学报,2017,43(7):974-982. |
| |
| 作者姓名： | 周可 李燕 王世明 崔国庆 杨正林 何光华 凌英华 赵芳明 |
| |
| 作者单位： | 1 西南大学水稻研究所 / 转基因植物与安全控制重庆市市级重点实验室，重庆400716; 2 贵州省茶叶研究所，贵州贵阳550006 |
| |
| 基金项目： | 本研究由国家重点研发计划项目(SQ2016ZY03001818)的“水稻杂种优势利用创新技术研究”课题(2016YFD0101107)和重庆市重点实验室能力提升项目(cstc2014pt-sy80001)资助。 |
| |
| 摘 要： | 花青素是广受人们喜爱的植物色素,在食品加工、杂种纯度鉴定中具有重要的作用。本研究鉴定了一个以日本晴为受体、优良紫鞘恢复系R225为供体亲本的紫鞘水稻染色体片段代换系Z519。Z519共含有16个代换片段,分布于除第10染色体外的其他11条染色体,平均长度为6.85 Mb。Z519在芽鞘3 mm时鞘尖呈现紫色,其后在叶鞘、叶缘、茎维管束和柱头等部位出现紫色线条,而日本晴各部位均为绿色。Z519叶鞘中花青素含量极显著高于日本晴,剑叶中没有显著差异。与受体日本晴相比,Z519的株高显著降低,千粒重、主穗总粒数和实粒数显著增加,有效穗数、主穗长和结实率无显著差异。进一步以日本晴与Z519杂交产生的F1和F2群体对紫鞘基因进行了遗传分析和分子定位。该紫鞘表型受显性单基因控制,位于第1染色体In Del标记L03和SSR标记L01之间37.8 kb的区域,被命名为PSH1。对该区间进行候选基因预测和测序,Z519在一个编码质体ATP/ADP转运蛋白的LOC_Os01g45910基因第一外显子的第238~252碱基的GTG重复区又多插入了GTG 3个碱基,导致增加了一个甘氨酸。q RT-PCR结果进一步表明其表达量在Z519中明显降低,初步确定LOC_Os01g45910是PSH1的候选基因。该研究为PSH1调控花青素的分子机制奠定了良好基础。
|
| 关 键 词： | 水稻 染色体片段代换系 紫鞘PSH1 基因定位 |
| 收稿时间： | 2016-11-25 |
Identification of Rice Chromosome Segment Substitution Line Z519 with Purple Sheath and Candidate Gene Analysis of PSH1 |
| |
| ZHOU Ke,LI Yan,WANG Shi-Ming,CUI Guo-Qing,YANG Zheng-Lin,HE Guang-Hua,LING Ying-Hua,ZHAO Fang-Ming.Identification of Rice Chromosome Segment Substitution Line Z519 with Purple Sheath and Candidate Gene Analysis of PSH1[J].Acta Agronomica Sinica,2017,43(7):974-982. |
| |
| Authors: | ZHOU Ke LI Yan WANG Shi-Ming CUI Guo-Qing YANG Zheng-Lin HE Guang-Hua LING Ying-Hua ZHAO Fang-Ming |
| |
| Institution: | 1.Rice Research Institute, Southwest University / Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Chongqing 400716, China;2.Tea Research Institute of Guizhou Province, Guiyang 550006, China |
| |
| Abstract: | Anthocyanins as plant pigments are widely liked by people and play a very important role in food processing and hybrid purity identification. Here, a rice chromosome segment substitution line (CSSL) Z519 with purple sheath was identified deriving from recipient Nipponbare and donor R225. Z519 contained 16 substitution segments with 6.85 Mb of average length, which were distributed on 11 chromosomes of rice except the 10th chromosome. The bud sheath of Z519 began to appear the purple color stripes when it was about 3 mm long. Then the purple stripes displayed on sheaths, leaf margins, vascular bundles of stem and stigmas. While all parts of Nipponbare were green. Anthocyanin content in leaf sheath of Z519 was significantly higher than that of Nipponbare, whereas no significant difference was in flag leaf. Compared with Nipponbare, plant height of Z519 was significantly decreased, spikelets number and grain number of main panicle, and 1000-grain weight of Z519 were significantly increased. There was no significant difference between Z519 and Nipponbare in the other traits such as panicle number, main panicle length and seed-setting rate. Then, F2 recessive populations from the cross of Nipponbare and Z519 were used for genetic analysis and gene mapping of the purple sheath. The purple sheath in Z519 was controlled by a single dominant gene, named as PSH1, which was mapped on the chromosome 1 between InDel marker L03 and SSR marker L01 with the physical distance of 37.8 kb. By sequencing and gene-predicting in the region, Z519 had three bases (GTG) insertion in the GTG repeat area of the 238th?252th base in the first exon compared with Nipponbare, which resulted in increasing a Gly amino acid. Furthermore, the expression of LOC_Os01g45910 was obviously decreased in Z519 by qRT-PCR analysis. Thus, LOC_Os01g45910 was preliminary identified as the candidate gene of PSH1. The results lay a good foundation for studying molecular mechanisms of regulating anthocyanin by PSH1. |
| |
| Keywords: | Rice (Oryza sativa L ) Chromosome segment substitution line (CSSL) Purple sheath PSH1 Gene mapping |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《作物学报》浏览原始摘要信息 |
| 点击此处可从《作物学报》下载免费的PDF全文 |
|
