| 小麦茎秆断裂强度相关性状QTL的连锁和关联分析 |
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| 引用本文： | 刘凯,邓志英,张莹,王芳芳,刘佟佟,李青芳,邵文,赵宾,田纪春,陈建省.小麦茎秆断裂强度相关性状QTL的连锁和关联分析[J].作物学报,2017,43(4):483-495. |
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| 作者姓名： | 刘凯 邓志英 张莹 王芳芳 刘佟佟 李青芳 邵文 赵宾 田纪春 陈建省 |
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| 作者单位： | 山东农业大学农学院小麦品质育种研究室/作物生物学国家重点实验室，山东泰安271018 |
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| 基金项目： | 本研究由山东省自然科学基金项目(2015ZRB01179),“小麦重要品质性状形成和改良的分子基础”育种专项和山东省种质资源创制课题资助。 |
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| 摘 要： | 小麦茎秆断裂强度与倒伏特性关系密切,并对产量有很大影响。本研究旨在解析茎秆断裂强度的遗传机制,开发与该性状紧密连锁/关联的分子标记。利用山农01-35′藁城9411重组自交系(RIL)群体(含173个F8:9株系)和由205个品种(系)构成的自然群体,借助90 k小麦SNP基因芯片、DArT芯片及传统分子标记技术,在2个环境中对两群体的茎秆断裂强度相关性状进行连锁分析和全基因组关联分析。利用已构建的高密度连锁图谱,在4B染色体的TDURUM_CONTIG63670_287–IACX557和EX_C101685–RAC875_C27536等区段上,检测到9个控制小麦茎秆断裂强度、株高、茎秆第2节间充实度、茎秆第2节壁厚相关性状的加性QTL,可解释表型变异9.40%~36.30%。同时,利用包含24 355个SNP位点的复合遗传图谱,在自然群体中检测到37个与茎秆断裂强度相关性状(P0.0001)的标记,分别位于1A、1B、2B、2D、3A、3B、4A、4B、5A、5B、5D、6B、7A、7B和7D染色体,可解释表型变异7.76%~36.36%。在4B染色体上,以连锁分析检测到控制茎秆断裂强度的RAC875_C27536与关联分析检测到的Tdurum_contig4974_355标记,在复合遗传图谱上的距离为6.7 cM,说明该区段存在控制小麦茎秆断裂强度的重要基因。
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| 关 键 词： | 普通小麦 QTL定位 连锁分析 全基因组关联分析 茎秆断裂强度 |
| 收稿时间： | 2016-05-27 |
Linkage Analysis and Genome-Wide Association Study of QTLs Controlling Stem-Breaking-Strength-Related Traits in Wheat |
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| LIU Kai,DENG Zhi-Ying,ZHANG Ying,WANG Fang-Fang,LIU Tong-Tong,LI Qing-Fang,SHAO Wen,ZHAO Bin,TIAN Ji-Chun,CHEN Jian-Sheng.Linkage Analysis and Genome-Wide Association Study of QTLs Controlling Stem-Breaking-Strength-Related Traits in Wheat[J].Acta Agronomica Sinica,2017,43(4):483-495. |
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| Authors: | LIU Kai DENG Zhi-Ying ZHANG Ying WANG Fang-Fang LIU Tong-Tong LI Qing-Fang SHAO Wen ZHAO Bin TIAN Ji-Chun CHEN Jian-Sheng |
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| Institution: | Group of Wheat Quality Breeding, College of Agronomy, Shandong Agricultural University / State Key Laboratory of Crop Biology, Tai’an 271018, China |
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| Abstract: | Stem strengthhas close relationship with lodging character, thereby, affects final yield in wheat. The objectives of this study were to unravel the genetic mechanism of stem-breaking-strength-related traits and find molecular markers closely linked or associated with these traits. We tried to map the stem-breaking-strength-related QTLs through linkage analysis using the RIL population consisting of 173 F8:9 lines derived from Shannong 01-35 ´ Gaocheng 9411) and associationanalysis using a nature population consisting of 2015 wheat varieties. Both populations were planted in two environments and genetically screened with the 90 k SNP array, DArT technology, and traditional molecular markers.By means of the existing high-density genetic map, nine additive QTLs were detectedin different regions onchromosome 4B, such asDURUM_CONTIG63670_287–IACX557 and EX_C101685–RAC875_C27536, which controlled stem-breaking strength, plant height, filling degree of the second internode and culm wall thickness of the second internode and explained 9.40–36.30% of the phenotypic variations.By means of a composite map (containing 24,355 SNPs) based on the IlluminaInfinium assay, a total of 37 SNPs were found in the natural population to be associated withstem-breaking-strength-relatedtraits (P<0.0001). These SNPswere distributed on chromosomes1B, 2B, 2D, 3A, 3B, 4B, 5A, 5D, 6B, 7A, 7B, and 7D and explained 10.70%–36.30% of the phenotypic variations.Thegenetic distance between RAC875_C27536 detected throughlinkage analysis and Tdurum_contig4974_355 detected through genome-wide association analysis was 6.7cM in thecomposite map, indicatingthe presence of importantgenes controlling stem strengthin this region. |
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| Keywords: | Common wheat QTL mapping Linkage analysis Genome-wide association study (GWAS) Stem-breaking strength |
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