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稻属间杂种花粉植株的细胞遗传学研究
引用本文:颜辉煌,胡慧英.稻属间杂种花粉植株的细胞遗传学研究[J].作物学报,1997,23(6):689-692.
作者姓名:颜辉煌  胡慧英
作者单位:中国水稻研究所,浙江杭州,310006
基金项目:中国农业科学院院长基金,国家自然科学基金资助项目
摘    要:以栽培稻02428与紧穗野生稻间不育F1杂种(2n=24,AC)为材料进行花约培养,共接种花约32688个,获得再生绿苗13株及白化苗两株,愈伤诱导率与分化率分别为0.14%和34.78%。有9朱花粉绿苗长至成熟,均表现长芒、紫柱头、强感光及易落粒等野生亲本的特性。细胞学结果进一步表明,其中4株为二倍体(2n=24),形态上琚的F1杂种相似,花粉母细胞中期Ⅰ亦只能见到少数二价体;另5株则为四倍体(

关 键 词:栽培稻  野生稻  种间杂种  花粉植株  染色体组成
收稿时间:1996-04-22
修稿时间:1996-07-20

Cytogenetical Studies on the Pollen-derived Plants from Interspecific Hybrid in Genus Oryza
Yan Huihuang,Hu Huiying,Zhang Xueqin,Zhang Zhitao Tian Shulan,Xiong Zhenmin,Min Shaokai.Cytogenetical Studies on the Pollen-derived Plants from Interspecific Hybrid in Genus Oryza[J].Acta Agronomica Sinica,1997,23(6):689-692.
Authors:Yan Huihuang  Hu Huiying  Zhang Xueqin  Zhang Zhitao Tian Shulan  Xiong Zhenmin  Min Shaokai
Institution:China National Rice Research Institute, Hangzhou, 310006
Abstract:Anther culture of a male-sterile interspecific hybrid between Oryza sativa vs. 02428 and O. eichingeri was attempted. From the 32688 anthers cultured, a total of 13 regenerated green plants and two albinos were eventually obtained, with the rate of callus induction and that of differentiation being 0. 14% and 34. 78%, respectively. All 9 green plants surviving to maturity showed traits of the wild species, including long awn, purple stigma, high photo-sensitivity and easy-shattering. They were further cytologically identified to be either diploids(2n = 24)or tetraploids(2n=48), in which 4 infertile diploids were similar to the original F1 hybrid morphologically and had also only a few bivalents at MI in PMCs, while two amphiploids with low self fertility and BPH resistance were, however, selected from the remained 5 tetraploid plants. It is therefore inferred that the F1 hybrid may have produced functional unreduced gametes from which the green plants originated.
Keywords:Oryza sativa  Oryza eichingeri  Interspecific hybrid  Pollen-derived plants  Chromosome composition
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