| 干旱锻炼对B73自交后代当代干旱胁迫记忆基因表达及其启动子区DNA甲基化的影响 |
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| 引用本文： | 王霞,尹晓雨,于晓明,刘晓丹.干旱锻炼对B73自交后代当代干旱胁迫记忆基因表达及其启动子区DNA甲基化的影响[J].作物学报,2022(5). |
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| 作者姓名： | 王霞 尹晓雨 于晓明 刘晓丹 |
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| 作者单位： | 吉林农业科技学院 |
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| 基金项目： | 吉林省教育厅“十三五”科学技术项目(JJKH20190972KJ);吉林农业科技学院青年基金项目(吉农院合字[2018]第1001号)资助。 |
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| 摘 要： | 植物可能会记住已有的逆境刺激,且这种记忆可以跨代遗传。为研究G0代干旱锻炼对玉米B73 G1代当代干旱胁迫记忆基因表达的影响及其启动子区DNA甲基化的影响,实验用20%PEG-6000模拟干旱条件,选取在玉米和拟南芥已知的当代均具有干旱胁迫记忆特性的6个基因为研究对象,利用qRT-PCR技术分析这些基因的表达水平变化,并进一步利用重亚硫酸盐测序PCR技术分析在2个世代中表达差异最显著基因启动子区的DNA甲基化率变化规律。结果表明:干旱胁迫上调了6个当代胁迫记忆基因的表达水平,均呈+/+模式,同当代多次干旱胁迫时表达水平变化趋势一致,且G1代表达水平显著高于G0代;干旱胁迫降低了GRMZM2G088396基因启动子区的甲基化水平,检测区1两个世代DNA甲基化率的降低主要由CHG和CHH甲基化水平降低造成,检测区2 DNA甲基化率的降低主要是由CG和CHH甲基化水平降低造成,G1代2个检测区总胞嘧啶甲基化率均显著低于G0代,说明G0代干旱锻炼使G1代GRMZM2G088396基因启动子区的DNA甲基化修饰产生了可遗传的变异,它可能直接参与GRMZM2G088396基因的表达。
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| 关 键 词： | 干旱锻炼 B73 干旱胁迫记忆基因 跨代记忆 QRT-PCR 重亚硫酸盐测序PCR技术 |
Effects of drought hardening on contemporary expression of drought stress memory genes and DNA methylation in promoter of B73 inbred progeny |
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| WANG Xia,YIN Xiao-Yu,Yu Xiao-Ming,LIU Xiao-Dan.Effects of drought hardening on contemporary expression of drought stress memory genes and DNA methylation in promoter of B73 inbred progeny[J].Acta Agronomica Sinica,2022(5). |
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| Authors: | WANG Xia YIN Xiao-Yu Yu Xiao-Ming LIU Xiao-Dan |
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| Institution: | (College of Jilin Agriculture Science and Technology,Jilin 132101,Jilin,China) |
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| Abstract: | Plants may remember the existing stresses,and this memory can be passed down across generations.To study the effects of drought hardening in G0 generation on the contemporary drought stress memory genes’expression and DNA methylation in promoter region in G1 generation,20%PEG-6000 was used to simulate drought condition,qRT-PCR was used to analyze the expression level changes of six genes with drought-stressed memory function in maize and Arabidopsis.The changes of DNA methylation rates in the promoter regions of the most differentially expressed gene was analyzed by bisulfite sequencing technology.The results showed that the expression of six genes were up-regulated under drought stress in a+/+mode,and the expression level of G1 generation was significantly higher than that of G0 generation.Drought stress reduced the DNA methylation’level in the promoter region of GRMZM2G088396 gene.The reduction of methylation in the detection region 1 of two generations was mainly caused by the reduction of CHG and CHH methylation level,the decrease of methylation in the detection region 2 was mainly caused by the decrease of CG and CHH methylation level.The total cytosine methylation rate of G1 generation was significantly lower than that of G0 generation,suggesting that drought exercise in G0 generation resulted in heritable variation of DNA methylation in the promoter region of GRMZM2G088396 gene in G1 generation,which may be directly involved in the expression of GRMZM2G088396 gene. |
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| Keywords: | drought hardening B73 drought stress memory gene transgenerational memory qRT-PCR bisulfite sequencing PCR |
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