Genetic diversity of new maize hybrids based on SSR markers as compared with other molecular and biochemical markers |
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| Authors: | Kamal Foud Abdellatif Yehia Abdallah Khidr |
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| Institution: | 1.Plant Biotechnology Dept., Genetic Engineering and Biotechnology, Research Institute (GEBRI),Minoufya University,Sadat City,Egypt |
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| Abstract: | Genetic diversity of four new yellow single crosses, five new yellow three-way crosses, and five yellow inbred lines of maize
(Zea mays L.) was studied using different molecular (SSR, ISSR, and RAPD) and biochemical markers (seed storage protein content). All
markers were able to clearly separate the inbred lines in one cluster from the different types of hybrids. The correlation
among the different types of molecular markers was moderately high according to the Mantel’s test (e.g. 0.67 between SSR and
ISSR, 0.42 between SSR and RAPD, and 0.65 between ISSR and RAPD), indicating that the three techniques are efficient for evaluating
genetic diversity in the maize genotypes. The correlation of biochemical markers (seed storage protein SDS-PAGE) with SSR,
ISSR, and RAPD markers was 0.61, 0.74, and 0.48, respectively. It can be concluded that both molecular and biochemical markers
are efficient to study the genetic diversity in maize. Among the different types of molecular markers, SSR is a more accurate
marker-type because of its co-dominance and stability of results. It can also be said that biochemical and molecular markers
are positively correlated and the correlation ranged between moderate to high. This could suggest using both marker types,
separately or together, for genetic diversity studies in maize. |
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