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| 转核酸酶barnase基因对小麦育性的影响 | |
| 引用本文： | 王勇,林忠平,徐惠君,李景富.转核酸酶barnase基因对小麦育性的影响[J].作物杂志,2011(5):27-30. |
| 作者姓名： | 王勇 林忠平 徐惠君 李景富 |
| 作者单位： | 1. 东北农业大学园艺学院,150030,黑龙江哈尔滨 2. 北京大学生命科学学院,100871,北京 3. 中国农业科学院作物科学研究所,100081,北京 |
| 基金项目： | 黑龙江省高校重点实验室开放基金项目(GS2009004); 东北农业大学创新团队课题(CX7002-4-2) |
| 摘 要： | 以小麦品系pm35为材料,利用基因枪法将核酸酶barnase基因导入小麦,获得12株阳性苗。至开花期,有7株表现为花粉明显减少,经花粉染色及花粉萌发率测定显示,该7株转基因小麦存在不同程度的花粉败育现象。进一步计算结实率发现,其结实率都明显下降,其中转基因植株10-11的结实率仅为3.43%。 |
| 关 键 词： | 小麦 核酸酶基因 花粉活力 结实率 雄性不育 |
Effect of Barnase on Fertility Expression in Wheat |
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| Wang Yong,Lin Zhongping,Xu Huijun,Li Jingfu.Effect of Barnase on Fertility Expression in Wheat[J].Crops,2011(5):27-30. | |
| Authors: | Wang Yong Lin Zhongping Xu Huijun Li Jingfu |
| Institution: | Wang Yong1,Lin Zhongping2,Xu Huijun3,Li Jingfu1(1College of Horticulture,Northeast Agricultural University,Harbin 150030,Heilongjiang,2College of Life Science,Peking University,Beijing 100871,3Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081,China) |
| Abstract: | Barnase gene was transferred into a wheat line pm35 by gene gun bombardment and 12 positive seedings were obtained.At flowering stage,the fertility of pollens of 7 plants reduced significantly through pollen staining and pollen germination test.The seed-setting rate decreased obviously,with the least of 3.43%(the 10-11 line). |
| Keywords: | Wheat Barnase gene Pollen vigor Seed-setting rate Male sterility |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |